Description
Human Apoptosis Signaling Array (19 targets) (SARB0022)
The Human Apoptosis Signaling Array (SARB0022) offered by AssayGenie is a powerful tool for researchers studying apoptosis and cell death pathways. This array contains 19 key targets involved in apoptosis signaling, allowing for the simultaneous detection and analysis of multiple proteins in a single experiment.With targets such as Bcl-2, caspase-3, and TNF receptor superfamily members, this array provides comprehensive coverage of the apoptotic signaling pathway. The array is compatible with human samples and can be used with a variety of biological samples, including cell lysates and tissue homogenates.
Using this array, researchers can gain valuable insights into the regulation of apoptosis and identify key proteins involved in cell death pathways. This information can be crucial for understanding the mechanisms underlying diseases such as cancer, neurodegenerative disorders, and autoimmune conditions.Overall, the Human Apoptosis Signaling Array (SARB0022) is a valuable tool for researchers seeking to unravel the complexities of apoptosis signaling and its role in health and disease.
| Product Sku: | SARB0022 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 19 |
| Gene Symbols: | AKT1, ATM, BAD, CASP3, CASP7, CHEK1, CHEK2, EIF2A, HSPB1, MAP3K7|MAPK1, MAPK3, MAPK8, NFKBIA, PARP1, PSMD9, RELA, SMAD2, TP53 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | Akt Signaling / HIF-1 alpha Signaling / IGF Signaling / JAK/STAT Signaling / MAPK Signaling / mTOR Signaling / NFkB Signaling / p53 Signaling / PI3K-AKT Signaling / TGF-beta Signaling / Wnt/beta-Catenin Signaling |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Akt (P-Ser473) (PKB (P-Ser473))
| ATM (P-Ser1981)
| BAD (P-Ser112)
| Caspase-3 (Cleaved Asp175)
| Caspase-7 (Cleaved Asp198)
|
CHK1 (P-Ser296)
| CHK2 (P-Thr68)
| eIF-2a (P-Ser52)
| ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
| HSP27 (P-Ser82)
|
IKBa (P-Ser32)
| JNK (P-Thr183/Tyr185)
| NFKBP65 (P-Ser536)
| PARP1 (Cleaved Asp214/Gly215)
| p27 (P-Thr198)
|
P53 (P-Ser15)
| SMAD2 (Ser245/250/255)
| TAK1 (P-Ser412)
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human Apoptosis Signaling Antibody Array C1 Membranes
- Blocking Buffer
- Detection Antibody Cocktail
- 1,000X HRP-Anti-Rabbit IgG Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- 100x Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated anti-IgG |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
