The Human CAV-1 (Caveolin-1) CLIA Kit is a reliable and accurate tool for the detection of Caveolin-1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.Caveolin-1 is a key protein involved in cell signaling, membrane trafficking, and lipid regulation. Its dysregulation has been linked to various diseases including cancer, cardiovascular disorders, and metabolic conditions, highlighting its significance as a biomarker for studying disease mechanisms and potential therapeutic interventions.
With the Human CAV-1 CLIA Kit, researchers can confidently measure Caveolin-1 levels in human samples, providing valuable insights into disease pathways and facilitating the development of innovative treatment strategies.
Product Name:
Human Cav-1 (Caveolin-1) CLIA Kit
SKU:
HUES00443
Target:
Human Cav-1 (Caveolin-1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Cav-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Cav-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Cav-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Cav-1. You can calculate the concentration of Human Cav-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
83-96
84-97
Average (%)
94
90
91
1:4
Range (%)
88-100
86-101
85-98
Average (%)
94
93
92
1:8
Range (%)
84-99
91-104
90-104
Average (%)
91
97
96
1:16
Range (%)
89-101
93-107
86-101
Average (%)
95
99
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
96
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
85-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
187.9
489.06
1495.25
175.33
534.89
1462.98
Standard deviation
15.6
49.64
153.86
21.21
53.76
143.96
C V (%)
8.3
10.15
10.29
12.1
10.05
9.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Cav-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Cav-1 in samples. No significant cross-reactivity or interference between Human Cav-1 and analogues was observed.
