Human CETP (Cholesteryl Ester Transfer Protein) CLIA Kit
The Human CETP (Cholesteryl Ester Transfer Protein) CLIA Kit is specifically designed for the precise measurement of CETP levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.CETP is a key protein involved in lipid metabolism, playing a vital role in the transfer of cholesteryl esters between lipoproteins. Dysregulation of CETP has been linked to a variety of metabolic disorders, including cardiovascular diseases and dyslipidemia.
By measuring CETP levels, researchers can gain valuable insights into these conditions and potentially develop innovative therapeutic strategies.Overall, the Human CETP CLIA Kit is an indispensable tool for investigating the role of CETP in various physiological and pathological processes, providing researchers with valuable data to advance their research efforts.
Product Name:
Human CETP (Cholesteryl Ester Transfer Protein) CLIA Kit
SKU:
HUES00460
Target:
Human CETP (Cholesteryl Ester Transfer Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CETP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CETP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CETP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CETP. You can calculate the concentration of Human CETP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
96-111
101-117
Average (%)
96
103
107
1:4
Range (%)
86-100
90-103
85-98
Average (%)
92
95
90
1:8
Range (%)
99-115
87-101
99-113
Average (%)
105
94
107
1:16
Range (%)
89-103
87-99
96-108
Average (%)
95
93
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
96-112
104
Cell culture media (n=5)
96-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
208.23
512.08
1563.1
194.82
547.85
1624.86
Standard deviation
17.14
54.38
104.57
23.05
64.76
155.99
C V (%)
8.23
10.62
6.69
11.83
11.82
9.6
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CETP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CETP in samples. No significant cross-reactivity or interference between Human CETP and analogues was observed.
