Human HABP1/C1QBP (Hyaluronan Binding Protein 1) CLIA Kit
The Human HABP1 (C1QBP) Hyaluronan Binding Protein 1 CLIA Kit is a cutting-edge assay designed for the precise measurement of HABP1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and selectivity, ensuring robust and consistent results for a variety of research applications.HABP1, also known as C1QBP, is a vital protein involved in hyaluronan binding and cell signaling. It plays a crucial role in various physiological processes and has been implicated in diseases such as cancer, inflammation, and autoimmune disorders.
By accurately measuring HABP1 levels, researchers can gain valuable insights into the role of this protein in disease pathogenesis and potentially identify new therapeutic targets.The Human HABP1 (C1QBP) Hyaluronan Binding Protein 1 CLIA Kit is a valuable tool for studying the function of HABP1 and its potential implications in disease progression. Its high sensitivity and specificity make it an excellent choice for researchers looking to uncover the intricate role of HABP1 in cellular processes and disease development.
Product Name:
Human HABP1/C1QBP (Hyaluronan Binding Protein 1) CLIA Kit
SKU:
HUES01284
Target:
Human HABP1/C1QBP (Hyaluronan Binding Protein 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HABP1/C1QBP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HABP1/C1QBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HABP1/C1QBP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HABP1/C1QBP. You can calculate the concentration of Human HABP1/C1QBP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
84-96
92-104
Average (%)
100
91
99
1:4
Range (%)
88-104
85-97
88-100
Average (%)
96
90
95
1:8
Range (%)
87-99
103-119
99-114
Average (%)
94
110
107
1:16
Range (%)
93-104
99-112
97-113
Average (%)
98
106
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
94
EDTA plasma (n=5)
94-110
101
Cell culture media (n=5)
94-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.39
290.08
811.44
105.22
297.49
850.24
Standard deviation
8.95
27.47
53.72
12.25
23.77
67.51
C V (%)
8.92
9.47
6.62
11.64
7.99
7.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HABP1/C1QBP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HABP1/C1QBP in samples. No significant cross-reactivity or interference between Human HABP1/C1QBP and analogues was observed.
