The Human NGF (Nerve Growth Factor) ELISA Kit is specifically designed for the quantitative detection of NGF levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results for various research purposes.NGF is a key protein that plays a vital role in the growth and maintenance of nerve cells, making it a valuable biomarker for studying neurodevelopmental disorders, neurodegenerative diseases, and neurological conditions.
By accurately measuring NGF levels, researchers can gain valuable insights into the underlying mechanisms of these conditions and potentially develop new therapeutic strategies.Overall, the Human NGF ELISA Kit is a valuable tool for researchers looking to investigate the role of NGF in various physiological and pathological processes, ultimately leading to a better understanding of nerve cell growth and function.
Product Name:
Human NGF (Nerve Growth Factor) ELISA Kit
SKU:
HUES02278
Target:
Human NGF (Nerve Growth Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NGF. You can calculate the concentration of Human NGF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
100-115
95-113
Average (%)
103
105
103
1:4
Range (%)
88-101
82-95
83-94
Average (%)
93
88
89
1:8
Range (%)
93-108
79-91
89-100
Average (%)
100
85
94
1:16
Range (%)
88-100
81-96
86-98
Average (%)
93
88
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
87-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.95
103.15
378.79
50.87
111.32
379.86
Standard deviation
2.77
4.32
14.39
2.93
6.37
15.92
C V (%)
5.66
4.19
3.8
5.76
5.72
4.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NGF in samples. No significant cross-reactivity or interference between Human NGF and analogues was observed.
