Human PDCD1LG1 (Programmed Cell Death Protein 1 Ligand 1) CLIA Kit
The Human PDCD1LG1 (Programmed Cell Death Protein 1 Ligand 1) CLIA Kit is a versatile tool designed for the accurate detection of PDCD1LG1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.PDCD1LG1, also known as PD-L1, is a key protein involved in modulating immune responses and maintaining immune tolerance.
Its dysregulation has been implicated in various diseases, including cancer and autoimmune disorders, making it a valuable biomarker for research and potential therapeutic development.With the Human PDCD1LG1 CLIA Kit, researchers can gain valuable insights into the role of PDCD1LG1 in disease progression and immune regulation, leading to advancements in diagnostics and treatment strategies.
Product Name:
Human PDCD1LG1 (Programmed Cell Death Protein 1 Ligand 1) CLIA Kit
SKU:
HUES00877
Target:
Human PDCD1LG1 (Programmed Cell Death Protein 1 Ligand 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PD-L1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PD-L1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PD-L1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PD-L1. You can calculate the concentration of Human PD-L1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
99-113
85-95
Average (%)
98
107
90
1:4
Range (%)
100-118
101-115
96-109
Average (%)
108
109
103
1:8
Range (%)
102-116
99-114
87-98
Average (%)
110
107
92
1:16
Range (%)
98-110
92-107
83-96
Average (%)
104
99
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-111
106
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
97-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.78
231.27
957.43
95.27
212.79
943.65
Standard deviation
10.08
20.31
109.91
12.28
18.11
63.22
C V (%)
9.62
8.78
11.48
12.89
8.51
6.7
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PD-L1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PD-L1 in samples. No significant cross-reactivity or interference between Human PD-L1 and analogues was observed.
