Human RAR alpha (Retinoic Acid Receptor Alpha) CLIA Kit
The Human RAR Alpha (Retinoic Acid Receptor Alpha) CLIA Kit is a precise and reliable tool for measuring the levels of RAR Alpha in human samples such as serum, plasma, and cell culture supernatants. This CLIA kit offers high sensitivity and specificity, allowing for accurate and reproducible results for various research applications.RAR Alpha is a crucial nuclear receptor that plays a key role in mediating the effects of retinoic acid in gene transcription, cell differentiation, and development. Dysregulation of RAR Alpha has been implicated in various diseases, including cancer, skin disorders, and developmental abnormalities.
Therefore, studying RAR Alpha levels using this CLIA kit can offer valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human RAR Alpha CLIA Kit from AssayGenie provides researchers with a powerful tool for investigating the role of RAR Alpha in health and disease, offering a deeper understanding of its function and potential clinical implications.
Product Name:
Human RAR alpha (Retinoic Acid Receptor Alpha) CLIA Kit
SKU:
HUES01255
Target:
Human RAR alpha (Retinoic Acid Receptor Alpha)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human RARα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RARα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RARα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human RARα. You can calculate the concentration of Human RARα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
101-117
87-100
Average (%)
96
107
92
1:4
Range (%)
88-101
88-101
91-108
Average (%)
95
93
99
1:8
Range (%)
92-105
95-110
101-119
Average (%)
99
103
109
1:16
Range (%)
99-113
96-111
88-100
Average (%)
105
104
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
92-107
100
Cell culture media (n=5)
101-119
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.74
174.25
966.98
97.46
172.24
884.5
Standard deviation
7.98
14.31
76.29
8.79
16.9
87.92
C V (%)
8.08
8.21
7.89
9.02
9.81
9.94
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human RARα concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RARα in samples. No significant cross-reactivity or interference between Human RARα and analogues was observed.
