Mouse ESM1 (Endothelial Cell Specific Molecule 1) CLIA Kit
The Mouse ESM1 (Endothelial Cell Specific Molecule 1) CLIA Kit is a highly reliable and sensitive assay designed for the accurate detection of ESM1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers excellent specificity and precision, ensuring consistent and reproducible results that are essential for a variety of research applications.ESM1, also known as endocan, is a protein that is specifically expressed in endothelial cells and plays a critical role in regulating inflammation and angiogenesis. Elevated levels of ESM1 have been associated with various diseases, including cancer, cardiovascular disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.
Overall, the Mouse ESM1 CLIA Kit provides researchers with a powerful tool for investigating the role of ESM1 in disease pathogenesis and assessing its potential as a therapeutic target. With its high sensitivity and accuracy, this kit is an essential addition to any laboratory conducting research in the fields of angiogenesis, inflammation, and endothelial cell biology.
Product Name:
Mouse ESM1 (Endothelial Cell Specific Molecule 1) CLIA Kit
SKU:
MOES00023
Target:
Mouse ESM1 (Endothelial Cell Specific Molecule 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ESM1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ESM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ESM1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ESM1. You can calculate the concentration of Mouse ESM1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
98-114
87-102
Average (%)
90
105
94
1:4
Range (%)
97-109
103-116
102-115
Average (%)
103
109
109
1:8
Range (%)
100-111
90-103
93-106
Average (%)
106
96
99
1:16
Range (%)
102-118
85-97
89-100
Average (%)
108
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
102-117
108
Cell culture media (n=5)
89-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.06
73.85
242.66
23.26
77.93
263.96
Standard deviation
2.06
8.18
18.18
2.82
8.0
21.25
C V (%)
8.93
11.08
7.49
12.12
10.27
8.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ESM1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ESM1 in samples. No significant cross-reactivity or interference between Mouse ESM1 and analogues was observed.
