Description
Canine Interleukin Array (11 targets) (SARB0009)
The Canine Interleukin Array - 11 Targets (SARB0009) offered by Assay Genie is a high-quality research tool for studying canine immune responses. This array allows for the simultaneous detection of 11 different interleukins, key signaling molecules involved in inflammation and immune regulation, in canine samples.With a focus on the canine immune system, this array provides researchers with valuable insights into the cytokine profile of their samples, making it ideal for studies in veterinary immunology, infectious diseases, and cancer research.
The array is easy to use, with clear results that can be analyzed quantitatively to understand the immune response dynamics in canine models.Whether investigating the immune response to pathogens, evaluating the efficacy of vaccines, or studying the role of inflammation in canine diseases, the Canine Interleukin Array - 11 Targets (SARB0009) is a versatile tool that can accelerate your research efforts in canine immunology. Trust Assay Genie for reliable and accurate results in your canine immune profiling studies.
| Product Sku: | SARB0009 |
| Size: | 2, 4, or 8 |
| Species Detected: | Canine |
| Number of Targets Detected: | 11 |
| Gene Symbols: | CXCL8, IL10, IL12B, IL17A, IL1B, IL2, IL6 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
| IL-1 alpha (IL-1 F1) | IL-1 beta (IL-1 F2)
| IL-1 Ra (IL-1 F3) | IL-10
| IL-12 p40
|
| IL-13 | IL-17A
| IL-2
| IL-21 | IL-6
|
IL-8 (CXCL8)
| ||||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Canine Interleukin Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
