Description
Glucose Quick Test Strips (BA0197) (BA0197)
The Glucose Quick Test Strips (SKU: BA0197) provide a rapid, instrument-free way to estimate glucose concentrations in food, beverage and biological samples. Glucose is present in the food industry both as a natural component and as an additive, valued for its effects on taste, texture and colour as well as its role as a preservative. These strips are based on glucose dehydrogenase-catalysed oxidation of glucose, in which the NADH formed reduces a chromogenic reagent to give a colour whose intensity is directly proportional to the glucose concentration. The result is read simply by comparing the developed reaction pad against a reference chart and multiplying by the dilution factor used. No expensive laboratory equipment is required and the entire procedure can be completed in around fifteen minutes. The kit is well suited to on-site screening of a wide range of sample types.
| Product Name: | Glucose Quick Test Strips (BA0197) |
| SKU: | BA0197 |
| Detection Method: | Visual (colorimetric test strip). Based on glucose dehydrogenase-catalysed oxidation of glucose, in which the NADH formed reduces a chromogenic reagent. The intensity of the product colour is directly proportional to the glucose concentration in the sample and is read by comparison against a reference chart. |
| Detection Range: | Semi-quantitative measurement of glucose in the sample |
| Sample Type: | Food and beverage samples (e.g. wine, beer, fruit juice, milk, yoghurt) and biological samples such as serum and urine |
| Species Reactivity: | All |
| Assay Time: | Approximately 15 minutes |
| Kit Size: | 10 tests |
| Equipment Required: | Benchtop |
| Storage: | Store at room temperature. Keep strips dry and out of direct sunlight. |
| Shelf Life: | 6 months upon receipt |
| Shipping: | Room Temperature |
These test strips estimate glucose semi-quantitatively using glucose dehydrogenase-catalysed oxidation of glucose, in which the NADH formed reduces a chromogenic reagent. After a sample is developed in the supplied development tube, a strip is dipped, colour is allowed to develop for five minutes and the reaction pad is compared against the provided glucose chart. The reading is multiplied by the dilution factor (e.g. 5x, 21x or 210x) to obtain the glucose concentration in the original sample.
- Fast and convenient. Results in approximately 15 minutes.
- No expensive laboratory equipment required.
- Suitable for a wide range of food, beverage and biological samples.
- Direct determination of glucose concentrations in food and beverage samples as well as biological samples such as serum and urine.
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Unscrew the cap of one of the Sample Development tubes. |
| 2 | For samples requiring a 21x dilution, transfer 20 µL of sample into the Sample Development tube using a 20 µL transfer pipette or pipettor. For samples requiring a 5x dilution, transfer 100 µL of sample using a 100 µL transfer pipette or pipettor. For samples requiring a 210x dilution, first prepare a 10x dilution by transferring 100 µL of sample into a Sample Dilution Tube containing 900 µL of distilled water, mix, then transfer 20 µL of this diluted sample into a Sample Development tube. |
| 3 | Replace the cap, close securely and invert the vial a couple of times to mix. |
| 4 | Unscrew the Sample Development Tube cap and dip in one of the test strips, fully submerging the reaction pad. Leave submerged for 5 seconds, then remove and shake to remove any clinging drops. |
| 5 | Let the colour develop on the strip for 5 minutes. |
| 6 | Compare the colour of the reaction pad with the provided Glucose Chart on the test strip bag and multiply the concentration by the dilution used (i.e. 5x, 21x or 210x). |
Read the glucose concentration by comparing the developed reaction pad against the provided Glucose Chart, then multiply the chart value by the dilution factor used (5x, 21x or 210x) to obtain the glucose concentration in the sample.
| Component | Quantity | Storage |
| Test Strips | 10 | Room Temperature |
| Sample Development Tubes (400 µL Development Reagent per tube) | 10 | Room Temperature |
| Instruction Manual | 1 | Room Temperature |
