The Human CT (Calcitonin) CLIA Kit is a highly sensitive and specific assay designed for the quantitative measurement of calcitonin levels in human serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, making it suitable for various research applications.Calcitonin is a vital hormone involved in calcium and phosphate metabolism, regulating bone formation and breakdown.
Abnormal calcitonin levels are associated with conditions such as osteoporosis, hypercalcemia, and thyroid disorders, making it a valuable biomarker for diagnosing and monitoring these conditions.With its reliable performance and ease of use, the Human CT (Calcitonin) CLIA Kit is an essential tool for researchers and healthcare professionals studying bone health, endocrine disorders, and other related fields.
Product Name:
Human CT (Calcitonin) CLIA Kit
SKU:
HUES00123
Target:
Human CT (Calcitonin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CT. You can calculate the concentration of Human CT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
94-109
100-114
Average (%)
100
101
105
1:4
Range (%)
88-101
84-98
102-114
Average (%)
93
91
108
1:8
Range (%)
101-120
95-113
88-102
Average (%)
109
103
95
1:16
Range (%)
95-110
94-108
89-100
Average (%)
100
101
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
103
EDTA plasma (n=5)
94-109
99
Cell culture media (n=5)
95-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.36
63.17
208.88
24.39
66.25
203.02
Standard deviation
2.87
7.11
13.77
2.21
6.81
23.16
C V (%)
11.78
11.26
6.59
9.06
10.28
11.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CT in samples. No significant cross-reactivity or interference between Human CT and analogues was observed.
