The Human IL32 CLIA Kit is specifically designed for the quantitative measurement of Interleukin 32 (IL32) levels in human serum, plasma, and cell culture supernatants. This highly sensitive and specific kit provides accurate and reproducible results, making it an excellent tool for a variety of research applications.IL32 is a key cytokine that plays a crucial role in inflammatory responses and immune regulation. Elevated levels of IL32 have been implicated in various inflammatory conditions, autoimmune diseases, and cancer, making it a valuable biomarker for studying these diseases and developing targeted therapies.
With its advanced technology and reliable performance, the Human IL32 CLIA Kit from Assay Genie offers researchers a powerful tool for studying the role of IL32 in health and disease. Order now to advance your research and discover new insights into the complex pathways regulated by IL32.
Product Name:
Human IL-32 CLIA Kit
SKU:
HUES00175
Target:
Human IL-32
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-32. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-32 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-32, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-32. You can calculate the concentration of Human IL-32 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
99-111
93-105
Average (%)
109
105
99
1:4
Range (%)
91-105
86-98
94-107
Average (%)
97
91
100
1:8
Range (%)
98-111
92-104
99-112
Average (%)
104
97
105
1:16
Range (%)
95-109
98-113
101-117
Average (%)
100
105
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
104
EDTA plasma (n=5)
86-97
92
Cell culture media (n=5)
83-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.96
50.03
241.26
27.2
49.4
219.06
Standard deviation
2.15
4.68
15.63
2.65
4.46
22.56
C V (%)
8.61
9.35
6.48
9.74
9.03
10.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-32 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-32 in samples. No significant cross-reactivity or interference between Human IL-32 and analogues was observed.
