Description
Pyruvate Quick Test Strips (BA0202) (BA0202)
The Pyruvate Quick Test Strips (SKU: BA0202) provide a rapid, instrument-free method for estimating pyruvate concentrations in beverage and biological samples. Pyruvate is a key intermediate in cellular metabolism that can be converted to carbohydrates, fatty acids, energy, alanine or ethanol depending on the pathway, and in the beverage industry its levels are monitored during alcoholic fermentation in the production of beer, wine and spirits. Abnormal pyruvate levels have also been linked to various liver diseases and metabolic disorders. These strips are based on pyruvate oxidase-catalysed oxidation of pyruvate, in which peroxidase reduces the peroxide formed and oxidises a chromogenic reagent to give a blue product proportional to the pyruvate concentration. The developed reaction pad is compared against a reference chart and multiplied by the dilution factor, with results obtained in around fifteen minutes.
| Product Name: | Pyruvate Quick Test Strips (BA0202) |
| SKU: | BA0202 |
| Detection Method: | Visual (colorimetric test strip). Based on pyruvate oxidase-catalysed oxidation of pyruvate, in which peroxidase reduces the peroxide formed and oxidises a chromogenic reagent. The intensity of the blue product is directly proportional to the pyruvate concentration in the sample and is read by comparison against a reference chart. |
| Detection Range: | Semi-quantitative measurement of pyruvate. Detection limits (pre-dilution): approximately 100 µM (8.7 mg/L) for 2x dilutions, 250 µM (22 mg/L) for 5x dilutions and 450 µM (39 mg/L) for 9x dilutions |
| Sample Type: | Beverage samples such as wine, beer and fruit juice, and biological samples such as serum, plasma and urine |
| Species Reactivity: | All |
| Assay Time: | Approximately 15 minutes |
| Kit Size: | 10 tests |
| Equipment Required: | Benchtop |
| Storage: | Store at room temperature. Keep strips dry and out of direct sunlight. |
| Shelf Life: | 6 months upon receipt |
| Shipping: | Room Temperature |
These test strips estimate pyruvate semi-quantitatively using pyruvate oxidase-catalysed oxidation of pyruvate, in which peroxidase reduces the peroxide formed and oxidises a chromogenic reagent to produce a blue product. After a sample is diluted in the supplied dilution tube, a strip is dipped, colour is allowed to develop for five minutes and the tan reaction pad is compared against the provided pyruvate chart. The reading is multiplied by the dilution factor (e.g. 2x, 5x or 9x) to obtain the pyruvate concentration in the original sample.
- Fast and convenient. Results in approximately 15 minutes.
- No expensive laboratory equipment required.
- Suitable for beverage and biological samples including wine, beer, serum and plasma.
- Direct determination of pyruvate concentrations in beverage samples such as wine and beer and biological samples such as serum, plasma and urine.
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Unscrew the cap of one of the Sample Dilution tubes. |
| 2 | For samples requiring a 9x dilution, transfer 50 µL of sample into a Sample Dilution tube using a 50 µL transfer pipette or pipettor. For samples requiring a 5x dilution, transfer 100 µL of sample using a 100 µL transfer pipette. For samples requiring a 2x dilution, transfer 400 µL of sample using a 400 µL transfer pipette. |
| 3 | Replace the cap, close securely and invert the vial a couple of times to mix the diluted sample. |
| 4 | Unscrew the cap and dip in one of the test strips, fully submerging the tan reaction pad. Leave submerged for 5 seconds, then remove and shake to remove any clinging drops. |
| 5 | Let the colour develop on the strip for 5 minutes. |
| 6 | Compare the colour of the reaction pad with the provided Pyruvate Chart on the test strip bag and multiply the concentration by the dilution used (i.e. 2x, 5x or 9x) to determine the pyruvate concentration in the sample. |
Read the pyruvate concentration by comparing the developed reaction pad against the provided Pyruvate Chart, then multiply the chart value by the dilution factor used (2x, 5x or 9x) to obtain the pyruvate concentration in the sample.
| Component | Quantity | Storage |
| Test Strips | 10 | Room Temperature |
| Sample Dilution Tubes (400 µL H2O per tube) | 10 | Room Temperature |
| Instruction Manual | 1 | Room Temperature |
