Description
L-Lactic Acid Test Strips (BA0176) (BA0176)
The L-Lactic Acid Test Strips (SKU: BA0176) provide a rapid, direct and equipment-free method for the semi-quantitative determination of L-lactic acid concentrations in food and beverage samples as well as biological samples. L-lactic acid, or L-lactate, is generated by lactate dehydrogenase under hypoxic or anaerobic conditions and is added to many foods and beverages to impart a tart flavour; increased levels in milk, egg and fruit juices can indicate spoilage, while in the wine industry rising L-lactic acid and falling L-malic acid are monitored during malolactic fermentation. The strips are based on L-lactate dehydrogenase catalysed oxidation of L-lactate, in which the NADH formed reduces a chromogenic reagent, and the intensity of the product colour is directly proportional to the L-lactate concentration in the sample. Sample treatment and assay can be completed in under 15 minutes without expensive laboratory equipment. The strips are intended for research use only.
| Product Name: | L-Lactic Acid Test Strips (BA0176) |
| SKU: | BA0176 |
| Detection Method: | Visual |
| Detection Range: | Semi-quantitative measurement between 0-360 mg/L (undiluted) L-lactic acid |
| Sample Type: | Wine, beer, milk and other food and beverage samples; biological samples such as urine and blood |
| Species Reactivity: | All |
| Assay Time: | Approximately 15 min |
| Kit Size: | 10 per pack |
| Equipment Required: | Microplate reader |
| Storage: | Room Temperature (Sample Development Tubes at 4C or below for long-term storage) |
| Shelf Life: | 6 months after receipt |
| Shipping: | Room Temperature |
Rapid visual test strips for the semi-quantitative determination of L-lactic acid in food, beverage and biological samples. The strips rely on L-lactate dehydrogenase catalysed oxidation of L-lactate, in which the NADH formed reduces a chromogenic reagent; the intensity of the developed colour is directly proportional to L-lactate concentration and is compared against a supplied colour chart. The strips provide a semi-quantitative measurement between 0 and 360 mg/L (undiluted) and the assay is completed in under 15 minutes.
- Fast and sensitive; uses 20 or 100 uL sample with semi-quantitative measurement between 0-360 mg/L (undiluted) L-lactic acid
- Convenient; no expensive laboratory equipment is needed
- Sample treatment and assay can be performed in under 15 minutes
- Rapid, direct determination of L-lactic acid in food and beverage samples such as wine, beer and milk
- Determination of L-lactic acid in biological samples such as urine and blood
- Monitoring of malolactic fermentation in wine production
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Dilute samples as appropriate: wine 21x, other acidic samples (fruit juice, beer, etc.) 5x, and homogenised milk 2x. Unscrew the cap of one Sample Development Tube. |
| 2 | Using the appropriate transfer pipette, add the required sample volume to the Sample Development Tube (20 uL for a 21x dilution, 100 uL for a 5x dilution or 400 uL for a 2x dilution). |
| 3 | Replace the cap, securely close the vial and invert it a couple of times to mix the diluted sample. |
| 4 | Unscrew the cap and dip one test strip in, fully submerging the yellow reaction pad for 5 seconds; then remove and shake to remove any drops. |
| 5 | Allow the colour to develop on the strip for 5 minutes. |
| 6 | Compare the colour of the reaction pad with the provided L-Lactic Acid Chart and multiply the chart concentration by the dilution used (2, 5 or 21). |
Compare the developed colour of the reaction pad against the provided L-Lactic Acid Chart to read the concentration, then multiply the chart value by the dilution factor used (2x, 5x or 21x) to obtain the L-lactate concentration of the sample.
| Component | Quantity | Storage |
| Test Strips | 10 | Room Temperature |
| Sample Development Tubes (400 uL Development Reagent per tube) | 10 | 4C or below for long-term storage |
| Instruction Manual | 1 | Room Temperature |
