Human Bid (BH3 Interacting Domain Death Agonist) CLIA Kit
The Human BID (BH3 Interacting Domain Death Agonist) CLIA Kit is a highly sensitive and specific assay designed to accurately measure BID levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for various research applications.BID is a key protein involved in cell death pathways, playing a crucial role in apoptosis regulation.
Dysregulation of BID has been linked to various diseases such as cancer and neurodegenerative disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its high-quality components and user-friendly design, the Human BID CLIA Kit from Assay Genie provides researchers with a powerful tool for investigating the role of BID in health and disease.
Product Name:
Human Bid (BH3 Interacting Domain Death Agonist) CLIA Kit
SKU:
HUES00388
Target:
Human Bid (BH3 Interacting Domain Death Agonist)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Bid. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Bid and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Bid, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Bid. You can calculate the concentration of Human Bid in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
89-103
89-101
Average (%)
107
95
95
1:4
Range (%)
88-99
86-99
98-116
Average (%)
94
91
107
1:8
Range (%)
94-110
90-101
86-96
Average (%)
101
96
91
1:16
Range (%)
99-114
86-97
98-112
Average (%)
106
91
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
99-112
105
Cell culture media (n=5)
97-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
192.26
496.06
1924.59
196.7
537.74
1826.19
Standard deviation
17.28
36.96
171.67
24.82
57.11
151.39
C V (%)
8.99
7.45
8.92
12.62
10.62
8.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Bid concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Bid in samples. No significant cross-reactivity or interference between Human Bid and analogues was observed.
