The Human Caspase-3 CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Caspase-3 levels in human samples, including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal choice for research applications in a variety of fields.Caspase-3 is a critical enzyme involved in apoptosis, the process of programmed cell death. Dysregulation of Caspase-3 activity has been implicated in various diseases, including cancer, Alzheimer's disease, and inflammatory conditions.
As such, this kit serves as a valuable tool for studying these conditions and developing potential therapeutic interventions.With its high sensitivity and specificity, the Human Caspase-3 CLIA Kit offers researchers a reliable method for quantifying Caspase-3 levels in human samples, providing valuable insights into the role of this enzyme in health and disease.
Product Name:
Human CASP3 (Caspase 3) CLIA Kit
SKU:
HUES00017
Target:
Human CASP3 (Caspase 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CASP3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CASP3. You can calculate the concentration of Human CASP3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
103-120
93-108
Average (%)
101
110
100
1:4
Range (%)
88-100
95-109
86-99
Average (%)
94
101
92
1:8
Range (%)
88-98
98-113
92-104
Average (%)
93
104
97
1:16
Range (%)
92-107
102-114
96-112
Average (%)
100
108
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-99
91
EDTA plasma (n=5)
87-101
94
Cell culture media (n=5)
98-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
238.2
766.53
1722.76
244.6
779.41
1628.67
Standard deviation
26.96
54.35
193.47
28.15
63.76
124.43
C V (%)
11.32
7.09
11.23
11.51
8.18
7.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CASP3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CASP3 in samples. No significant cross-reactivity or interference between Human CASP3 and analogues was observed.
