The Mouse AIF (Apoptosis-Inducing Factor) CLIA Kit is specifically designed for the quantitative detection of AIF levels in mouse samples. This kit offers high sensitivity and accuracy, providing researchers with reliable and reproducible results for their studies.AIF is a key protein involved in the induction of apoptosis, the programmed cell death process essential for maintaining tissue homeostasis and eliminating damaged cells. Dysregulation of AIF has been implicated in various diseases, including neurodegenerative disorders, cancer, and cardiovascular diseases, highlighting its significance as a potential therapeutic target.
With its advanced technology and high-performance capabilities, the Mouse AIF CLIA Kit is an invaluable tool for researchers investigating the role of AIF in disease pathogenesis, as well as for developing novel treatment strategies. Trust in this kit to deliver precise and detailed measurements of AIF levels in mouse samples, paving the way for groundbreaking discoveries in the field of apoptosis research.
Product Name:
Mouse AIF (Apoptosis Inducing Factor) CLIA Kit
SKU:
MOES00089
Target:
Mouse AIF (Apoptosis Inducing Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse AIF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AIF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse AIF. You can calculate the concentration of Mouse AIF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
103-120
95-106
Average (%)
96
109
101
1:4
Range (%)
101-119
84-99
101-116
Average (%)
109
91
107
1:8
Range (%)
87-99
93-110
92-108
Average (%)
92
100
99
1:16
Range (%)
98-114
85-95
87-101
Average (%)
106
90
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
103
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
97-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
21.5
35.62
189.01
23.06
37.33
192.35
Standard deviation
2.67
2.83
13.93
2.01
3.4
14.27
C V (%)
12.42
7.94
7.37
8.72
9.11
7.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse AIF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AIF in samples. No significant cross-reactivity or interference between Mouse AIF and analogues was observed.
