Based on the information from the provided URL, the Human DPP4 (Dipeptidyl Peptidase IV) CLIA Kit is a cutting-edge research tool designed for the precise measurement of DPP4 levels in human samples. This kit offers unparalleled sensitivity and specificity, guaranteeing accurate and consistent results for various research applications. DPP4 is a key enzyme involved in metabolism and immune regulation, playing a crucial role in conditions like diabetes, cardiovascular diseases, and inflammatory disorders.
By accurately quantifying DPP4 levels in biological samples, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets. The Human DPP4 CLIA Kit is a valuable tool for scientists and clinicians alike, empowering them to delve deeper into the complex interplay of DPP4 in health and disease. With its advanced technology and reliable performance, this kit is an indispensable asset for any laboratory conducting research on DPP4-related pathways and diseases.
Product Name:
Human DPP4 (Dipeptidyl Peptidase IV) CLIA Kit
SKU:
HUES00056
Target:
Human DPP4 (Dipeptidyl Peptidase IV)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DPP4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DPP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DPP4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DPP4. You can calculate the concentration of Human DPP4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
101-116
89-100
Average (%)
97
107
95
1:4
Range (%)
90-103
101-117
101-116
Average (%)
97
107
109
1:8
Range (%)
91-106
89-104
99-114
Average (%)
97
95
106
1:16
Range (%)
96-111
100-115
88-103
Average (%)
104
106
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-111
102
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
88-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
237.67
673.6
2467.34
224.47
722.14
2334.61
Standard deviation
30.28
50.39
201.09
25.77
56.98
157.35
C V (%)
12.74
7.48
8.15
11.48
7.89
6.74
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DPP4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DPP4 in samples. No significant cross-reactivity or interference between Human DPP4 and analogues was observed.
