The Human IL-22 CLIA Kit is a cutting-edge assay designed for the precise measurement of IL-22 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable tool for various research applications.IL-22 is a key cytokine that plays a crucial role in immune response and inflammation.
Its dysregulation has been linked to various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and potentially identifying new therapeutic targets. The Human IL-22 CLIA Kit provides researchers with a reliable means to quantify IL-22 levels, advancing our understanding of its role in health and disease.
Product Name:
Human IL-22 CLIA Kit
SKU:
HUES00102
Target:
Human IL-22
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-22. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-22 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-22, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-22. You can calculate the concentration of Human IL-22 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
94-109
101-117
Average (%)
102
101
109
1:4
Range (%)
97-110
89-102
86-102
Average (%)
102
96
93
1:8
Range (%)
93-107
90-100
86-99
Average (%)
100
95
91
1:16
Range (%)
83-98
91-105
100-114
Average (%)
90
96
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
91-104
97
Cell culture media (n=5)
100-113
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.99
67.26
239.53
26.27
62.18
231.15
Standard deviation
2.73
5.43
18.76
3.17
6.23
22.58
C V (%)
11.38
8.07
7.83
12.07
10.02
9.77
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-22 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-22 in samples. No significant cross-reactivity or interference between Human IL-22 and analogues was observed.
