The Human MSM-beta (Microseminoprotein beta) CLIA Kit is a highly specific and sensitive assay designed for the reliable detection of MSM-beta levels in human samples including serum, plasma, and cell culture supernatants. This advanced kit offers precise and reproducible results, making it an ideal tool for a wide range of research applications.MSM-beta is a key protein involved in various biological processes, including the regulation of immune responses and inflammatory pathways.
Dysregulation of MSM-beta has been linked to several diseases, including prostate cancer and inflammatory conditions, making it a valuable biomarker for studying these diseases and potentially developing new treatment strategies.With its high performance and accuracy, the Human MSM-beta CLIA Kit from AssayGenie is an essential tool for researchers looking to investigate the role of MSM-beta in health and disease. Order yours today and take your research to the next level.
Product Name:
Human MSM beta (Microseminoprotein Beta) CLIA Kit
SKU:
HUES00400
Target:
Human MSM beta (Microseminoprotein Beta)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MSMβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MSMβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MSMβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MSMβ. You can calculate the concentration of Human MSMβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
87-101
97-113
Average (%)
105
94
104
1:4
Range (%)
97-111
95-109
89-101
Average (%)
105
101
96
1:8
Range (%)
100-116
101-117
98-112
Average (%)
106
109
106
1:16
Range (%)
88-102
91-105
94-109
Average (%)
96
98
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
95
EDTA plasma (n=5)
97-112
105
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.07
75.59
297.62
37.22
81.64
312.54
Standard deviation
4.79
5.62
24.49
3.67
6.5
30.66
C V (%)
12.58
7.43
8.23
9.86
7.96
9.81
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MSMβ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MSMβ in samples. No significant cross-reactivity or interference between Human MSMβ and analogues was observed.
