The Human NEF3 (Neurofilament-3) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of NEF3 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.NEF3 is an important protein in the nervous system, specifically in the formation and maintenance of neuronal structure.
Abnormal levels of NEF3 have been associated with neurodegenerative disorders such as Alzheimer's and Parkinson's disease, making it a valuable biomarker for studying these conditions and potentially developing new therapeutic approaches.Get your Human NEF3 CLIA Kit now and unlock insights into neurodegenerative diseases with confidence and precision.
Product Name:
Human NEF3 (Neurofilament 3) CLIA Kit
SKU:
HUES00466
Target:
Human NEF3 (Neurofilament 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NEF3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NEF3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NEF3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NEF3. You can calculate the concentration of Human NEF3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-104
99-115
90-103
Average (%)
95
105
97
1:4
Range (%)
85-95
96-109
97-109
Average (%)
90
101
103
1:8
Range (%)
88-101
91-103
100-113
Average (%)
93
97
106
1:16
Range (%)
92-106
95-107
93-108
Average (%)
99
101
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
106
EDTA plasma (n=5)
97-110
103
Cell culture media (n=5)
96-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.47
104.0
346.57
39.81
105.51
359.61
Standard deviation
4.76
9.56
31.16
4.36
8.4
36.28
C V (%)
11.48
9.19
8.99
10.95
7.96
10.09
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NEF3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NEF3 in samples. No significant cross-reactivity or interference between Human NEF3 and analogues was observed.
