The Human PCDH15 (Protocadherin-15) CLIA Kit is a highly reliable and sensitive assay designed for the precise detection of PCDH15 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high specificity and accuracy, ensuring consistent and repeatable results for a variety of research applications.PCDH15, also known as Protocadherin-15, is a critical protein involved in cell adhesion and signaling. It plays a crucial role in maintaining the structure and function of inner ear hair cells, making it essential for auditory function.
Mutations in the PCDH15 gene can lead to various types of hearing loss, including Usher syndrome, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Human PCDH15 CLIA Kit is a powerful tool for researchers studying inner ear disorders, hearing loss, and related genetic conditions. Its high sensitivity and specificity make it an ideal choice for accurate and reliable quantification of PCDH15 levels in human samples.
Product Name:
Human PCDH15 (Protocadherin 15) CLIA Kit
SKU:
HUES00570
Target:
Human PCDH15 (Protocadherin 15)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PCDH15. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PCDH15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PCDH15, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PCDH15. You can calculate the concentration of Human PCDH15 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-106
102-119
89-104
Average (%)
97
109
96
1:4
Range (%)
102-116
92-108
87-103
Average (%)
109
98
94
1:8
Range (%)
97-111
84-100
92-108
Average (%)
103
91
99
1:16
Range (%)
95-108
101-113
99-110
Average (%)
100
106
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
107
EDTA plasma (n=5)
99-116
107
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
221.88
367.36
1530.19
204.35
397.2
1501.93
Standard deviation
28.07
41.62
114.61
24.3
44.53
134.57
C V (%)
12.65
11.33
7.49
11.89
11.21
8.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PCDH15 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PCDH15 in samples. No significant cross-reactivity or interference between Human PCDH15 and analogues was observed.
