The Human SLIT2 (Slit Homolog 2) CLIA Kit is specifically designed for the accurate detection of SLIT2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.SLIT2 is a key protein involved in cell migration, axon guidance, and angiogenesis.
It plays a critical role in various physiological processes, making it a valuable biomarker for studying conditions such as cancer, neurological disorders, and cardiovascular diseases. The Human SLIT2 CLIA Kit provides researchers with a powerful tool for investigating the role of SLIT2 in these conditions and developing potential therapies.
Product Name:
Human Slit2 (Slit Homolog 2) CLIA Kit
SKU:
HUES00554
Target:
Human Slit2 (Slit Homolog 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Slit. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Slit and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Slit, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human Slit. You can calculate the concentration of Human Slit in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-115
97-115
98-112
Average (%)
105
105
104
1:4
Range (%)
88-102
99-115
92-105
Average (%)
95
106
99
1:8
Range (%)
89-105
101-115
94-108
Average (%)
97
106
102
1:16
Range (%)
100-113
98-113
93-104
Average (%)
105
105
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
99-115
107
Cell culture media (n=5)
96-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.07
293.09
997.64
106.84
267.01
986.6
Standard deviation
11.86
31.24
98.27
10.73
23.28
97.67
C V (%)
11.97
10.66
9.85
10.04
8.72
9.9
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human Slit concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Slit in samples. No significant cross-reactivity or interference between Human Slit and analogues was observed.
