Human TGF- alpha (Transforming Growth Factor alpha) CLIA Kit
The Human TGF-Alpha (Transforming Growth Factor-Alpha) CLIA Kit is a powerful tool for accurately measuring TGF-Alpha levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reliable results, making it ideal for a variety of research applications.TGF-Alpha is a key signaling molecule involved in cell growth, differentiation, and development. It plays a crucial role in various biological processes including tissue repair, wound healing, and immune response. Dysregulation of TGF-Alpha has been linked to various diseases such as cancer, fibrosis, and inflammatory disorders, highlighting its importance as a biomarker for disease research and therapeutic development.
The Human TGF-Alpha CLIA Kit from Assay Genie provides researchers with a valuable tool for studying the role of TGF-Alpha in health and disease, ultimately advancing our understanding of complex biological processes and potential therapeutic targets. Visit our website to learn more about this innovative kit and its applications in biomedical research.
Product Name:
Human TGF- alpha (Transforming Growth Factor alpha) CLIA Kit
SKU:
HUES00902
Target:
Human TGF- alpha (Transforming Growth Factor alpha)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TGF-α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TGF-α. You can calculate the concentration of Human TGF-α in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
88-104
95-110
Average (%)
99
95
103
1:4
Range (%)
84-98
95-112
100-115
Average (%)
91
102
108
1:8
Range (%)
93-104
98-114
94-108
Average (%)
98
104
99
1:16
Range (%)
90-102
89-103
104-118
Average (%)
97
96
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
98
EDTA plasma (n=5)
85-95
90
Cell culture media (n=5)
89-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.2
44.69
220.02
23.08
44.34
237.52
Standard deviation
2.32
4.18
19.89
2.39
5.13
14.89
C V (%)
10.0
9.35
9.04
10.36
11.57
6.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TGF-α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TGF-α in samples. No significant cross-reactivity or interference between Human TGF-α and analogues was observed.
