The Human TNPO2 (Transportin 2) CLIA Kit is a cutting-edge assay designed for the accurate measurement of TNPO2 levels in human samples including serum, plasma, and cell culture supernatants. This highly sensitive and specific kit ensures reliable and reproducible results, making it an essential tool for researchers in various fields.TNPO2, also known as Transportin 2, plays a critical role in nucleocytoplasmic transport, mediating the transportation of proteins into the nucleus. Dysregulation of TNPO2 has been implicated in various diseases including cancer, infectious diseases, and neurological disorders, highlighting its importance as a potential biomarker for disease diagnosis and treatment development.
With its advanced technology and user-friendly protocols, the Human TNPO2 CLIA Kit offers researchers a valuable tool for studying the role of TNPO2 in disease pathogenesis and for identifying potential therapeutic targets. Unlock the potential of TNPO2 research with this innovative and reliable assay kit.
Product Name:
Human TNPO2 (Transportin 2) CLIA Kit
SKU:
HUES00858
Target:
Human TNPO2 (Transportin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TNPO2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNPO2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNPO2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TNPO2. You can calculate the concentration of Human TNPO2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-116
92-105
90-104
Average (%)
106
100
97
1:4
Range (%)
87-100
95-110
101-118
Average (%)
93
102
108
1:8
Range (%)
93-109
99-117
87-101
Average (%)
99
107
92
1:16
Range (%)
87-101
97-112
88-104
Average (%)
94
104
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
99
EDTA plasma (n=5)
101-115
107
Cell culture media (n=5)
85-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
217.83
443.46
1695.05
229.08
406.73
1546.39
Standard deviation
24.2
33.3
181.37
25.52
38.19
146.6
C V (%)
11.11
7.51
10.7
11.14
9.39
9.48
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TNPO2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNPO2 in samples. No significant cross-reactivity or interference between Human TNPO2 and analogues was observed.
