The Mouse BLC (B-Lymphocyte Chemoattractant) CLIA Kit is a cutting-edge diagnostic tool designed to accurately measure BLC levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and consistent results, making it a valuable asset for a variety of research applications.BLC is a key chemokine that plays a critical role in the immune response by attracting B-lymphocytes to sites of inflammation and infection.
By studying BLC levels, researchers can gain valuable insights into the functioning of the immune system and develop new strategies for treating conditions such as autoimmune disorders, allergies, and infectious diseases.Overall, the Mouse BLC CLIA Kit provides researchers with a reliable and efficient tool for studying the role of BLC in immune function and disease pathogenesis.
Product Name:
Mouse BLC (B-Lymphocyte Chemoattractant) CLIA Kit
SKU:
MOES00120
Target:
Mouse BLC (B-Lymphocyte Chemoattractant)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BLC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BLC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BLC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BLC. You can calculate the concentration of Mouse BLC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
93-106
85-97
Average (%)
107
100
90
1:4
Range (%)
86-99
97-110
89-102
Average (%)
92
103
96
1:8
Range (%)
101-116
92-104
94-108
Average (%)
106
97
100
1:16
Range (%)
88-104
98-112
101-117
Average (%)
95
106
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
100-117
106
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.51
51.11
235.03
27.17
50.74
212.28
Standard deviation
2.48
5.15
16.62
2.81
3.85
24.41
C V (%)
9.72
10.08
7.07
10.34
7.59
11.5
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse BLC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BLC in samples. No significant cross-reactivity or interference between Mouse BLC and analogues was observed.
