The Mouse ECF (CCL11/Eosinophil Chemotactic Factor) CLIA Kit is a powerful tool for detecting levels of ECF in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.ECF, also known as CCL11, is a critical chemokine involved in recruiting eosinophils to inflammatory sites and regulating immune responses. Elevated levels of ECF have been implicated in various inflammatory conditions, such as allergies, asthma, and autoimmune diseases.
By accurately measuring ECF levels, researchers can gain valuable insights into the mechanisms underlying these diseases and explore potential therapeutic strategies.Whether studying the role of ECF in immune responses or investigating its potential as a therapeutic target, the Mouse ECF CLIA Kit provides a reliable and efficient solution for precise quantification of this important chemokine in mouse samples.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ECF/CCL11. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ECF/CCL11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ECF/CCL11, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ECF/CCL11. You can calculate the concentration of Mouse ECF/CCL11 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
99-114
88-100
Average (%)
93
104
94
1:4
Range (%)
84-96
86-98
97-111
Average (%)
91
92
105
1:8
Range (%)
84-96
87-100
95-110
Average (%)
90
92
100
1:16
Range (%)
103-115
102-115
85-100
Average (%)
109
108
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
97-113
103
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.72
88.88
387.61
35.07
91.21
356.11
Standard deviation
3.93
6.54
41.24
2.87
8.34
36.54
C V (%)
10.7
7.36
10.64
8.18
9.14
10.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ECF/CCL11 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ECF/CCL11 in samples. No significant cross-reactivity or interference between Mouse ECF/CCL11 and analogues was observed.
