The Mouse SCF (Stem Cell Factor) CLIA Kit is specifically designed for the precise measurement of SCF levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for various research purposes.SCF, also known as c-kit ligand, is a vital cytokine that plays a critical role in hematopoiesis, stem cell maintenance, and immune responses. It regulates the proliferation, differentiation, and survival of various cell types, making it a crucial factor in developmental biology and oncology research.
With its reliable performance and broad applicability, the Mouse SCF (Stem Cell Factor) CLIA Kit is an indispensable tool for researchers studying hematopoiesis, stem cell biology, and immunology. Get reliable results and uncover new insights into SCF biology with this high-quality assay kit.
Product Name:
Mouse SCF (Stem Cell Factor) CLIA Kit
SKU:
MOES00340
Target:
Mouse SCF (Stem Cell Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse SCF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SCF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SCF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse SCF. You can calculate the concentration of Mouse SCF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-102
91-105
98-111
Average (%)
96
96
103
1:4
Range (%)
93-108
85-99
88-103
Average (%)
99
90
95
1:8
Range (%)
100-116
96-108
88-103
Average (%)
108
102
95
1:16
Range (%)
95-105
96-113
85-99
Average (%)
100
103
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-108
98
EDTA plasma (n=5)
98-113
104
Cell culture media (n=5)
87-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.02
76.44
173.36
21.69
69.33
160.56
Standard deviation
2.94
9.04
11.89
2.58
6.51
13.94
C V (%)
12.24
11.83
6.86
11.89
9.39
8.68
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse SCF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse SCF in samples. No significant cross-reactivity or interference between Mouse SCF and analogues was observed.
