The Rat BMP-2 (Bone Morphogenetic Protein-2) CLIA Kit is a specialized assay kit designed for the accurate detection of BMP-2 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing researchers with reliable and reproducible results for a variety of research applications.BMP-2 is a key protein involved in bone formation and regeneration, playing a critical role in skeletal development and repair.
Dysregulation of BMP-2 has been linked to conditions such as osteoporosis, bone fractures, and other skeletal disorders, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Rat BMP-2 CLIA Kit is an essential tool for researchers seeking to investigate the role of BMP-2 in bone biology and explore its potential applications in the treatment of musculoskeletal disorders.
Product Name:
Rat BMP-2 (Bone Morphogenetic Protein 2) CLIA Kit
SKU:
RTES00002
Target:
Rat BMP-2 (Bone Morphogenetic Protein 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat BMP-2. You can calculate the concentration of Rat BMP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
85-97
104-118
Average (%)
102
92
110
1:4
Range (%)
85-95
98-110
96-109
Average (%)
90
104
103
1:8
Range (%)
86-101
100-115
102-116
Average (%)
93
106
110
1:16
Range (%)
83-97
95-110
87-102
Average (%)
90
101
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
100
EDTA plasma (n=5)
91-104
98
Cell culture media (n=5)
95-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.34
157.34
439.66
49.44
144.52
449.65
Standard deviation
5.63
17.92
49.64
4.92
15.09
43.08
C V (%)
11.41
11.39
11.29
9.95
10.44
9.58
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat BMP-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat BMP-2 in samples. No significant cross-reactivity or interference between Rat BMP-2 and analogues was observed.
