The Rat MMP-8 (Matrix Metalloproteinase-8) CLIA Kit is a highly sensitive and specific assay designed for the quantitative detection of MMP-8 levels in rat serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it an excellent tool for researchers studying MMP-8's role in various physiological and pathological processes.MMP-8 is a member of the matrix metalloproteinase family, known for its involvement in tissue remodeling and inflammation. Elevated levels of MMP-8 have been associated with various conditions, including cancer, arthritis, and wound healing, highlighting its importance as a potential biomarker for disease progression and treatment monitoring.
By using the Rat MMP-8 CLIA Kit, researchers can accurately measure MMP-8 levels in rat samples, gaining valuable insights into its biological functions and potential therapeutic targets. This kit offers a convenient and efficient solution for studying MMP-8 dynamics in research settings, paving the way for advancements in various fields of study.
Product Name:
Rat MMP-8 (Matrix Metalloproteinase 8) CLIA Kit
SKU:
RTES00386
Target:
Rat MMP-8 (Matrix Metalloproteinase 8)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat MMP-8. You can calculate the concentration of Rat MMP-8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
92-107
97-111
Average (%)
105
99
104
1:4
Range (%)
102-116
85-96
92-106
Average (%)
109
91
99
1:8
Range (%)
103-115
91-104
98-111
Average (%)
109
96
105
1:16
Range (%)
86-96
86-100
93-108
Average (%)
91
93
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
105
EDTA plasma (n=5)
88-101
95
Cell culture media (n=5)
89-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.66
126.22
468.69
49.11
115.66
430.58
Standard deviation
5.82
9.95
31.59
4.04
10.49
47.84
C V (%)
12.75
7.88
6.74
8.23
9.07
11.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat MMP-8 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat MMP-8 in samples. No significant cross-reactivity or interference between Rat MMP-8 and analogues was observed.
