The Rat PCX (Podocalyxin) CLIA Kit is specifically designed for the quantification of podocalyxin levels in rat samples, including serum, plasma, and cell culture supernatants. This highly sensitive and accurate kit provides reliable and reproducible results, making it an invaluable tool for research in various fields.Podocalyxin, also known as PCX, is a key glycoprotein involved in cell adhesion and signaling, playing a crucial role in cell differentiation and migration. Elevated levels of podocalyxin have been linked to various diseases, including cancer and kidney disorders, making it an important biomarker for studying disease progression and potential therapeutic interventions.
With its high performance and easy-to-use format, the Rat PCX CLIA Kit is an essential tool for researchers studying podocalyxin and its role in disease development and progression. It provides a reliable and efficient method for accurate quantification of podocalyxin levels, enabling further insights into the molecular mechanisms underlying various pathological conditions.
Product Name:
Rat PCX (Podocalyxin) CLIA Kit
SKU:
RTES00459
Target:
Rat PCX (Podocalyxin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat PCX. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PCX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PCX, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat PCX. You can calculate the concentration of Rat PCX in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
91-108
92-104
Average (%)
100
98
97
1:4
Range (%)
100-116
87-103
101-114
Average (%)
106
94
107
1:8
Range (%)
99-115
92-106
95-108
Average (%)
107
99
102
1:16
Range (%)
100-117
99-113
96-110
Average (%)
106
105
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
94
EDTA plasma (n=5)
101-115
106
Cell culture media (n=5)
91-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.0
2.87
7.53
1.1
3.13
7.94
Standard deviation
0.12
0.27
0.62
0.1
0.36
0.5
C V (%)
12.0
9.41
8.23
9.09
11.5
6.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat PCX concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat PCX in samples. No significant cross-reactivity or interference between Rat PCX and analogues was observed.
