The Mouse AQP-1 (Aquaporin-1) CLIA Kit is specifically designed for the quantitative measurement of aquaporin-1 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for various research applications.Aquaporin-1 is a water channel protein that plays a critical role in regulating water transportation across cell membranes. Dysregulation of aquaporin-1 has been implicated in various diseases such as kidney disorders, brain edema, and cardiovascular conditions.
Hence, monitoring aquaporin-1 levels is essential for understanding the pathophysiology of these diseases and identifying potential therapeutic targets.With easy-to-follow protocols and quick assay procedures, the Mouse AQP-1 CLIA Kit is a valuable tool for researchers studying water homeostasis, fluid balance, and related cellular processes in mouse models. Get precise and insightful data with this advanced assay kit from Assay Genie.
Product Name:
Mouse AQP-1 (Aquaporin 1) CLIA Kit
SKU:
MOES00093
Target:
Mouse AQP-1 (Aquaporin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse AQP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AQP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AQP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse AQP-1. You can calculate the concentration of Mouse AQP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-117
95-109
100-112
Average (%)
109
103
106
1:4
Range (%)
92-103
89-106
90-103
Average (%)
97
97
95
1:8
Range (%)
100-115
86-101
99-115
Average (%)
105
92
106
1:16
Range (%)
98-114
92-105
90-105
Average (%)
105
97
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
97-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.02
284.17
778.16
92.04
279.0
707.23
Standard deviation
7.71
22.14
84.12
8.14
27.37
63.08
C V (%)
8.11
7.79
10.81
8.84
9.81
8.92
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse AQP-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse AQP-1 in samples. No significant cross-reactivity or interference between Mouse AQP-1 and analogues was observed.
