Mouse FABP5 (Fatty Acid Binding Protein 5, Epidermal) CLIA Kit
The Mouse FABP5 (Fatty Acid Binding Protein 5) CLIA Kit is specifically designed for the quantitative detection of FABP5 levels in mouse serum, plasma, and tissue homogenates. This kit utilizes a Chemiluminescent Immunoassay (CLIA) technique, providing high sensitivity and accuracy in measuring FABP5 concentrations.FABP5 is a key protein involved in lipid metabolism and transport, playing a critical role in various physiological processes such as skin development and maintenance. Abnormal FABP5 levels have been linked to diseases like diabetes, cancer, and inflammatory disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic strategies.
With its reliable performance and easy-to-use protocol, the Mouse FABP5 CLIA Kit is an essential tool for researchers studying lipid metabolism, skin biology, and related disorders in mouse models. Get accurate and reproducible results with this innovative kit from AssayGenie.
Product Name:
Mouse FABP5 (Fatty Acid Binding Protein 5, Epidermal) CLIA Kit
SKU:
MOES00254
Target:
Mouse FABP5 (Fatty Acid Binding Protein 5, Epidermal)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FABP5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FABP5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FABP5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FABP5. You can calculate the concentration of Mouse FABP5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
90-104
96-110
Average (%)
108
96
104
1:4
Range (%)
88-99
100-119
99-113
Average (%)
94
109
107
1:8
Range (%)
90-103
96-106
87-98
Average (%)
97
101
93
1:16
Range (%)
92-104
83-97
94-107
Average (%)
99
90
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
97
EDTA plasma (n=5)
95-106
100
Cell culture media (n=5)
91-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.61
288.63
734.19
108.87
300.01
669.32
Standard deviation
14.22
21.36
71.88
13.5
27.6
62.72
C V (%)
12.97
7.4
9.79
12.4
9.2
9.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FABP5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FABP5 in samples. No significant cross-reactivity or interference between Mouse FABP5 and analogues was observed.
