Recombinant Human Vitamin D-Binding Protein/GC Protein (His Tag) (RPES2584)

SKU:
RPES2584
€142

Description

ELISA Kit Technical Manual

Recombinant Human Vitamin D-Binding Protein/GC Protein (His Tag)

Background:Vitamin D-Binding Protein (DBP) is a member of the ALB/AFP/VDB family. DBP is a secreted protein and contains three albumin domains. The primary structure contains 28 cysteine residues forming multiple disulfide bonds. DBP acts as a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. DBP binds to vitamin D and its plasma metabolites and transports them to target tissues. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
Name:Recombinant Human Vitamin D-Binding Protein/GC Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES2584
Synonyms:Vitamin D-Binding Protein; DBP; VDB; Gc-Globulin; Group-Specific Component; GC
Species:Human
Expression host:Human Cells
Sequence:Leu17-Leu474
Accession:P02774
Mol Mass:52.3 kDa
AP Mol Mass:53 kDa
Tag:C-6His
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM PB,150mM NaCl,pH7.2.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q12-q13

Cellular Component: cytosol; extracellular region; extracellular space; lysosomal lumen

Molecular Function:actin binding; vitamin D binding

Biological Process: vitamin D metabolic process

Disease: Graves Disease, Susceptibility To, 1

NCBI Summary:The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]
UniProt Code:P02774
NCBI GenInfo Identifier:139641
NCBI Gene ID:2638
NCBI Accession:P02774.1
UniProt Secondary Accession:P02774,Q16309, Q16310, Q53F31, Q6GTG1, B4DPP2, D6RAK8
UniProt Related Accession:P02774
Molecular Weight:55,123 Da
NCBI Full Name:Vitamin D-binding protein
NCBI Synonym Full Names:GC, vitamin D binding protein
NCBI Official Symbol:GC
NCBI Official Synonym Symbols:DBP; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; HEL-S-51
NCBI Protein Information:vitamin D-binding protein
UniProt Protein Name:Vitamin D-binding protein
UniProt Synonym Protein Names:Gc protein-derived macrophage activating factor
Protein Family:GCR1-dependent translation factor
UniProt Gene Name:GC
UniProt Entry Name:VTDB_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
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