The XRCC4 Polyclonal Antibody (CAB18046) is a valuable tool for researchers studying XRCC4, a key protein involved in DNA double-strand break repair and maintenance of genomic stability. This antibody, produced in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications.XRCC4 is essential for the non-homologous end joining (NHEJ) pathway of DNA repair, playing a critical role in the repair of DNA damage caused by factors such as radiation and chemotherapy. Dysregulation of XRCC4 function has been implicated in various diseases, including cancer, highlighting the importance of studying this protein in a research setting.
By using the XRCC4 Polyclonal Antibody, researchers can accurately detect and analyze XRCC4 expression in various cell types, providing valuable insights into the molecular mechanisms of DNA repair and genomic stability. This antibody is an ideal tool for studies in areas such as oncology, genomics, and molecular biology, offering a reliable means of investigating the role of XRCC4 in health and disease.
Product Name:
[KO Validated] XRCC4 Rabbit Polyclonal Antibody
SKU:
CAB18046
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human XRCC4 (NP_001304941.1?).
The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternate transcript variants such as NM_022406 are unlikely to be expressed in some individuals due to a polymorphism (rs1805377) in the last splice acceptor site.
Purification Method:
Affinity purification
Gene ID:
7518
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and XRCC4 knockout (KO) HeLa cells, using [KO Validated] XRCC4 Rabbit pAb (CAB18046) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
