The SMAD7 Polyclonal Antibody (CAB16396) is a valuable tool for researchers studying the SMAD7 protein, a key regulator of the TGF-β signaling pathway. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting SMAD7 in human samples, making it an ideal choice for Western blot applications.SMAD7 is a critical player in the TGF-β signaling pathway, where it acts as a negative regulator by inhibiting the activity of SMAD2 and SMAD3. Dysregulation of SMAD7 has been linked to various diseases, including cancer, fibrosis, and autoimmune disorders, making it a compelling target for therapeutic interventions.
By utilizing the SMAD7 Polyclonal Antibody, researchers can accurately detect and quantify SMAD7 levels in different cell types and tissues, contributing to a better understanding of its role in disease development and progression. This antibody is a valuable tool for studies in molecular biology, oncology, and immunology, providing insights that may lead to the development of novel treatments targeting the TGF-β pathway.
Product Name:
Smad7 Rabbit Polyclonal Antibody
SKU:
CAB16396
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Smad7 (NP_005895.1).
The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4092
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse brain, using Smad7 Rabbit pAb (CAB16396) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
