Description
CHSY1 Antibody (PACO23415)
The CHSY1 Polyclonal Antibody (PAC023415) is a valuable tool for researchers studying the CHSY1 protein, involved in chondroitin sulfate synthesis. This antibody, produced in rabbits, is highly specific to human samples and is ideal for use in Western blot applications. By targeting the CHSY1 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it essential for studies in glycobiology and extracellular matrix research.CHSY1 is a crucial enzyme in the biosynthesis of chondroitin sulfate, a key component of the extracellular matrix involved in cell signaling and tissue structure.
Dysregulation of CHSY1 has been linked to various diseases, including cancer and developmental disorders, making it a promising target for therapeutic interventions. Understanding the function of CHSY1 is essential for unraveling its role in disease pathogenesis and developing targeted treatments to restore chondroitin sulfate homeostasis.
| Antibody Name: | CHSY1 Antibody (PACO23415) |
| Antibody SKU: | PACO23415 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000, IHC:1:50-1:100 |
| Species Reactivity: | Human |
| Immunogen: | Synthesized peptide derived from internal of human CHSY1. |
| Form: | Liquid |
| Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from HepG2 cells and K562 cells, using CHSY1 antibody. |
 | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CHSY1 antibody. |
| Background: | Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Involved in the negative control of osteogenesis likely through the modulation of NOTCH signaling. |
| Synonyms: | carbohydrate (chondroitin) synthase 1; chondroitin glucuronyltransferase II; chondroitin synthase; CHSS1; glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase 1 |
| UniProt Protein Function: | CHSY1: Has both beta-1,3-glucuronic acid and beta-1,4-N- acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Involved in the negative control of osteogenesis likely through the modulation of NOTCH signaling. Defects in CHSY1 are the cause of Temtamy preaxial brachydactyly syndrome (TPBS). A syndrome characterized by multiple congenital anomalies, mental retardation, sensorineural deafness, talon cusps of upper central incisors, growth retardation, and bilateral symmetric digital anomalies mainly in the form of preaxial brachydactyly and hyperphalangism. Belongs to the chondroitin N- acetylgalactosaminyltransferase family. |
| UniProt Protein Details: | Protein type:Transferase; EC 2.4.1.175; Membrane protein, integral; Glycan Metabolism - chondroitin sulfate biosynthesis; EC 2.4.1.226 Chromosomal Location of Human Ortholog: 15q26.3 Cellular Component: extracellular region; Golgi membrane; membrane Molecular Function:glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity; N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity Biological Process: chondroitin sulfate biosynthetic process; negative regulation of ossification Disease: Temtamy Preaxial Brachydactyly Syndrome |
| NCBI Summary: | This gene encodes a member of the chondroitin N-acetylgalactosaminyltransferase family. These enzymes possess dual glucuronyltransferase and galactosaminyltransferase activity and play critical roles in the biosynthesis of chondroitin sulfate, a glycosaminoglycan involved in many biological processes including cell proliferation and morphogenesis. Decreased expression of this gene may play a role in colorectal cancer, and mutations in this gene are a cause of temtamy preaxial brachydactyly syndrome. [provided by RefSeq, Dec 2011] |
| UniProt Code: | Q86X52 |
| NCBI GenInfo Identifier: | 116241296 |
| NCBI Gene ID: | 22856 |
| NCBI Accession: | Q86X52.3 |
| UniProt Secondary Accession: | Q86X52,Q6UX38, Q7LFU5, Q9Y2J5, |
| UniProt Related Accession: | Q86X52 |
| Molecular Weight: | 91,784 Da |
| NCBI Full Name: | Chondroitin sulfate synthase 1 |
| NCBI Synonym Full Names: | chondroitin sulfate synthase 1 |
| NCBI Official Symbol: | CHSY1Â Â |
| NCBI Official Synonym Symbols: | CHSY; CSS1; TPBS; ChSy-1Â Â |
| NCBI Protein Information: | chondroitin sulfate synthase 1 |
| UniProt Protein Name: | Chondroitin sulfate synthase 1 |
| UniProt Synonym Protein Names: | Chondroitin glucuronyltransferase 1; Chondroitin synthase 1; ChSy-1; Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase 1; N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 1; N-acetylgalactosaminyltransferase 1 |
| Protein Family: | Chondroitin sulfate synthase |
| UniProt Gene Name: | CHSY1Â Â |
| UniProt Entry Name: | CHSS1_HUMAN |
