The NEK11 Polyclonal Antibody (PAC015694) is a valuable tool for researchers studying NEK11, a key protein involved in cell cycle regulation and DNA damage response. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting NEK11 in human samples, making it suitable for use in Western blot and immunohistochemistry applications. By targeting the NEK11 protein, researchers can gain insights into its role in cellular processes such as cell division and DNA repair.
NEK11 is a crucial player in maintaining genomic stability and ensuring accurate cell division. Dysregulation of NEK11 has been linked to various diseases, including cancer, making it a promising target for therapeutic intervention. By studying the function of NEK11, researchers can uncover new pathways for potential drug targets and develop novel treatment strategies for cancer and other related disorders.
Antibody Name:
NEK11 Antibody (PACO15694)
Antibody SKU:
PACO15694
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:5000, WB:1:500-1:2000
Species Reactivity:
Human
Immunogen:
Fusion protein of human NEK11
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-2: A549 cells, 231 cells, Primary antibody: PACO15694(NEK11 Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute.
Background:
This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.
Synonyms:
NIMA-related kinase 11
UniProt Protein Function:
NEK11: Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation. Interacts with isoform 1 of NEK2. Poorly expressed in cerebellum, trachea, lung, appendix, and uterus. Autorepressed by intramolecular binding of the C-terminus which dissociates following phosphorylation by NEK2 isoform 1 in G1/S-arrested cells. NEK2 isoform 2 is largely not present in the nucleolus, and does not appear to phosphorylate NEK11. Activated in response to DNA damage. Inhibited by zinc. Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. 4 isoforms of the human protein are produced by alternative splicing.Protein type: Nucleolus; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Other group; NEK familyChromosomal Location of Human Ortholog: 3q22.1Cellular Component: nucleolus; nucleoplasmMolecular Function: ATP binding; protein binding; protein serine/threonine kinase activityBiological Process: G2/M transition DNA damage checkpoint; histone phosphorylation; intra-S DNA damage checkpoint; protein amino acid phosphorylation
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009]
