Description
PRSS3 Antibody (PACO33212)
The PRSS3 Polyclonal Antibody (PAC033212) is a valuable tool for researchers studying the PRSS3 protein, a serine protease involved in various physiological processes, including digestion and immune regulation. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications, enabling researchers to detect and analyze PRSS3 in different cell types.PRSS3, also known as Mesotrypsin, is an enzyme that plays a crucial role in protein degradation and has been implicated in various diseases, including cancer and inflammatory conditions.
By targeting PRSS3, researchers can gain insight into its function and potential therapeutic applications in these disease contexts. Understanding the role of PRSS3 is essential for developing targeted interventions to modulate its activity and potentially treat related medical conditions.
| Antibody Name: | PRSS3 Antibody (PACO33212) |
| Antibody SKU: | PACO33212 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Trypsin-3 protein (81-303AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot Positive WB detected in: U87 whole cell lysate All lanes: PRSS3 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 33, 29, 27, 26 kDa Observed band size: 27 kDa . |
 | IHC image of PACO33212 diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Digestive protease specialized for the degradation of trypsin inhibitors. |
| Synonyms: | Trypsin-3 (EC 3.4.21.4) (Brain trypsinogen) (Mesotrypsinogen) (Serine protease 3) (Serine protease 4) (Trypsin III) (Trypsin IV), PRSS3, PRSS4 TRY3 TRY4 |
| UniProt Protein Function: | Digestive protease specialized for the degradation of trypsin inhibitors. In the ileum, may be involved in defensin processing, including DEFA5. |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene is localized to the locus of T cell receptor beta variable orphans on chromosome 9. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2010] |
| UniProt Code: | P35030 |
| NCBI GenInfo Identifier: | 209572698 |
| NCBI Gene ID: | 5646 |
| NCBI Accession: | P35030.2 |
| UniProt Secondary Accession: | P35030,P15951, Q15665, Q5VXV0, Q6ISJ4, Q9UQV3, A8CED1 A8CED3, A9Z1Y4, E7ES07 |
| UniProt Related Accession: | P35030 |
| Molecular Weight: | 25,924 Da |
| NCBI Full Name: | Trypsin-3 |
| NCBI Synonym Full Names: | protease, serine 3 |
| NCBI Official Symbol: | PRSS3Â Â |
| NCBI Official Synonym Symbols: | T9; MTG; TRY3; TRY4; PRSS4Â Â |
| NCBI Protein Information: | trypsin-3 |
| UniProt Protein Name: | Trypsin-3 |
| UniProt Synonym Protein Names: | Brain trypsinogen; Mesotrypsinogen; Serine protease 3; Serine protease 4; Trypsin III; Trypsin IV |
| Protein Family: | |
| UniProt Gene Name: | PRSS3Â Â |
| UniProt Entry Name: | TRY3_HUMAN |
| Antibodies |
| PRSS3 Antibody (PACO01660) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
