The OCRL Polyclonal Antibody (CAB19976) is a valuable tool for researchers studying OCRL, a protein involved in the regulation of cell signaling and trafficking. This antibody, derived from rabbit immunoglobulins, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the OCRL protein, this antibody allows for the detection and analysis of OCRL levels in various cell types, making it ideal for investigations in cell biology and related fields.
OCRL is known for its role in cellular processes such as endocytosis and membrane trafficking, making it a crucial player in maintaining cellular homeostasis. Dysregulation of OCRL has been linked to diseases like Lowe syndrome and Dent2 disease, highlighting the importance of studying this protein in both normal and pathological conditions. By understanding the function of OCRL, researchers can gain insights into potential therapeutic targets for these disorders and other related conditions.
Product Name:
[KO Validated] OCRL Rabbit Polyclonal Antibody
SKU:
CAB19976
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human OCRL (NP_001578.2).
This gene encodes an inositol polyphosphate 5-phosphatase. This protein is involved in regulating membrane trafficking and is located in numerous subcellular locations including the trans-Golgi network, clathrin-coated vesicles and, endosomes and the plasma membrane. This protein may also play a role in primary cilium formation. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
4952
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and OCRL knockout (KO) 293T cells, using [KO Validated] OCRL Rabbit pAb (CAB19976) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
