The Phospho-MYH9 (S1943) Polyclonal Antibody (CABP0802) is specifically designed for research involving MYH9, a key protein involved in cell contraction and migration. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications, including Western blot and immunofluorescence.MYH9, also known as non-muscle myosin heavy chain IIA, is essential for cell movement and is implicated in various cellular processes such as cell adhesion, migration, and division. The phosphorylation of MYH9 at Ser1943 has been linked to its role in cell motility and contractility, making it a critical target for research in cell biology and cancer metastasis.
The Phospho-MYH9 (S1943) Polyclonal Antibody enables researchers to specifically detect and analyze the phosphorylated form of MYH9 in different cell types, providing valuable insights into its function and regulation. Studies using this antibody can contribute to a better understanding of MYH9-mediated cellular processes and potentially lead to the development of targeted therapies for diseases involving dysregulated cell movement and metastasis.
Product Name:
Phospho-MYH9-S1943 Rabbit Polyclonal Antibody
SKU:
CABP0802
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S1943 of human MYH9 (NP_002464.1).
This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness.
Purification Method:
Affinity purification
Gene ID:
4627
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Phospho-MYH9-S1943 Rabbit pAb (CABP0802) at 1:2000 dilution. 293T cell lysates were treated by CIP at 37°C for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
