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KO Validated Antibodies

Knock Out Validated Antibodies

Highly specific antibodies for consistent results!

Figure 1: Schematic representation of how KO validated antibodies are created using CRISPR technology.

How do we validate our Knockout Antibodies?

  • We have created hundreds of CRISPR Knockout cell lines by introducing frame-shift mutations into the genomic DNA of target cells using sgRNA/CAS9.

  • The CRISPR knockout cells are lysed and Western Blotting is performed using Wild-Type (WT) cells as a control.

  • The Western Blots are then probed using our premium antibodies. Visualization of decreased protein expression is indicative of highly specific antibodies (Knockout Validated Antibodies).

Popular Validated Antibody Targets

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Bcl10 Antibody [KO Validated]
Reactivity Human
Application WB
Research Area Cell Death
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CAPG Antibody [KO Validated]
Reactivity Human, Mouse, Rat
Application WB, IHC
Research Area Cell Biology
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Cytokeratin 8 Antibody [KO Validated]
Reactivity Human, Mouse
Application WB, IHC, IF
Research Area Immunology
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GIT1 Antibody [KO Validated]
Reactivity Human, Mouse, Rat
Application WB, IF
Research Area Signal Transduction
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EEF1B2 Antibody [KO Validated]
Reactivity Human, Mouse, Rat
Application WB, IHC, IF
Research Area Metabolism
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AK2 Antibody [KO Validated]
Reactivity Human, Mouse, Rat
Application WB, IHC
Research Area Cell Biology
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CDK8 Antibody [KO Validated]
Reactivity Human, Mouse, Rat
Application WB, IP
Research Area Epigenetics and Nuclear Signaling
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EGFR Antibody [KO Validated]
Reactivity Human, Mouse, Rat
Application WB, IHC, IF, IP
Research Area Immunology
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HDAC8 Antibody [KO Validated]
Reactivity Human, Mouse, Rat
Application WB, IHC, IF
Research Area Epigenetics and Nuclear Signaling
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NF2 Antibody [KO Validated]
Reactivity Human, Mouse, Rat
Application WB, IF, IP
Research Area Cell Biology

Looking for a particular antibody? Use our LiveChat to get in touch and one of our specialists will be happy to help!

What is KO Validation?

  • KO validation is a means by which antibody specificity is confirmed by testing antibodies in a knockout (KO) cell line.
  • Using KO cell lines (cells with no expression of target proteins) is the new gold standard to validate the specificity of antibodies.

  • If a signal is detected from a KO cell line, then the antibody is not highly specific. This is due to the antibody binding to a similar protein within the sample. Antibodies with high specificity on the other hand will not produce any signal.

  • KO validated antibodies must be shown to be specific, selective, and reproducible.

Figure 2: Immunohistochemistry of paraffin-embedded human colon using KO validated FASN antibody (CAB0462) at dilution of 1:100 (40x lens).

Figure 3: Immunofluorescence analysis of HeLa cells using KO validated KRT8 antibody (CAB1024) at dilution of 1:100. Blue: DAPI for nuclear staining.

Why is validation important?

  • Antibodies are commonly used research tools. These proteins are used routinely in experiments such as Western blot, ELISA, immunostaining (immunohistochemistry (IHC) and immunocytochemistry (ICC)), immunofluorescence, and flow cytometry.

  • For the findings of these experimental techniques to be reliable they must have an internally high standard of accuracy and precision.

  • This is why there is a need for highly specific antibodies that can ensure reproducibility of results.

Additional Resources