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[KO Validated] RhoGDI Rabbit Polyclonal Antibody (CAB13468)

SKU:
CAB13468
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
  • KO Validated Antibodies 1 Anti-ARHGDIA Antibody CAB13468KO Validated
  • KO Validated Antibodies 1 Anti-ARHGDIA Antibody CAB13468KO Validated
  • KO Validated Antibodies 1 Anti-ARHGDIA Antibody CAB13468KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] RhoGDI Rabbit Polyclonal Antibody
SKU:CAB13468
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human RhoGDI (NP_001172006.1).
Sequence:MAEQ EPTA EQLA QIAA ENEE DEHS VNYK PPAQ KSIQ EIQE LDKD DESL RKYK EALL GRVA VSAD PNVP NVVV TGLT LVCS SAPG PLEL DLTG DLES FKKQ SFVL KEGV EYRI KISF RVNR EIVS GMKY IQHT YRKG VKID KTDY MVGS YGPR AEEY EFLT PVEE APKG MLAR GSYS IKSR FTDD DKTD HLSW EWNL TIKK DWKD
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e; DI
Positive Sample:293T
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:23kDa
Observed MW:23kDa

This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants.

Purification Method:Affinity purification
Gene ID:396
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts from normal (control) and RhoGDI knockout (KO) 293T cells, using RhoGDI antibody (CAB13468) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

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