ANPEP
ANPEP (alanyl aminopeptidase, membrane), also known as aminopeptidase N, CD13, APN, gp150 and LAP1, is a membrane-bound zinc metalloprotease of the peptidase M1 family. It digests peptides at the cell surface and acts as a receptor for certain coronaviruses. Browse Assay Genie's current range of ANPEP research reagents below, or read on for the biology behind the target.
ANPEP encodes aminopeptidase N, a zinc-binding metalloprotease that belongs to the peptidase M1 family. It is found in the small-intestinal and renal microvillar membrane and in other plasma membranes. In the small intestine it contributes to the final digestion of peptides generated when gastric and pancreatic proteases hydrolyse dietary proteins. Its role in proximal tubular epithelial cells and other cell types is less clear, although the enzyme has been thought to take part in the metabolism of regulatory peptides in cell types including small intestinal and renal tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS.
The protein has a large extracellular carboxy-terminal domain that carries a pentapeptide consensus sequence typical of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical, which is why the ANPEP gene and protein appear in the literature under both names. Further aliases include LAP1, gp150, p150, AP-N, hAPN and PEPN. HGNC classifies the gene within the aminopeptidases, CD molecules and M1 metallopeptidases groups, and lists the earlier name alanyl (membrane) aminopeptidase.
ANPEP is also a virus-facing protein. This membrane-bound zinc metalloprotease serves as a receptor for the HCoV-229E alphacoronavirus and for other non-human coronaviruses, and UniProt notes that it also mediates human cytomegalovirus (HCMV) infection. The gene has been shown to promote angiogenesis, tumour growth and metastasis, and defects in it are associated with various types of leukaemia and lymphoma.
Aminopeptidase N in the small-intestinal microvillar membrane contributes to the final digestion of peptides produced by gastric and pancreatic proteases.
ANPEP is a receptor for HCoV-229E and other non-human coronaviruses, making it a focus for studies of viral attachment and entry.
The gene promotes angiogenesis, tumour growth and metastasis, and is linked to leukaemia and lymphoma, so it is studied in tumour biology.
UniProt reports that the protein also mediates human cytomegalovirus infection, giving a further route into host-pathogen interaction work.
ANPEP encodes aminopeptidase N, a membrane-bound zinc metalloprotease. In the small intestine it helps complete the digestion of peptides released by gastric and pancreatic proteases. It is also a receptor for HCoV-229E and other non-human coronaviruses, and it mediates HCMV infection.
Yes. Sequence comparisons with known zinc metalloproteinases showed that CD13 and aminopeptidase N are identical. Other names for the product of the ANPEP gene include LAP1, gp150, p150, AP-N, hAPN and PEPN, and the earlier HGNC name was alanyl (membrane) aminopeptidase.
NCBI Gene reports that ANPEP promotes angiogenesis, tumour growth and metastasis, and that defects in it are associated with various leukaemias and lymphomas. Orthologues named Anpep exist in mouse and rat; check the datasheet of any product for its validated reactivity.
Other aminopeptidases, CD molecules and coronavirus-receptor targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P15144; product specifications from Assay Genie datasheets.