Histone H3
Histone H3 is the substrate of haspin, the serine/threonine-protein kinase encoded by HASPIN (also known as GSG2). Haspin phosphorylates histone H3 at Thr-3 during mitosis, helping to position the chromosomal passenger complex at centromeres and to keep cell division on course. Browse Assay Genie's current range of Histone H3 research reagents below, or read on for the biology behind the target.
This page covers the histone H3 associated protein kinase, haspin, a serine/threonine-protein kinase that phosphorylates histone H3 at Thr-3 (H3T3ph) during mitosis. It is thought to act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres. Through this activity it supports proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.
The HASPIN gene encodes a member of the protein kinase superfamily, placed in the Ser/Thr protein kinase family and the haspin subfamily. HGNC classifies it in the histone H3 associated protein kinase family. NCBI Gene annotates the protein with ATP binding and histone H3T3 kinase activity, and links it to mitotic sister chromatid cohesion, mitotic spindle assembly checkpoint signalling and protein localisation to the centromeric region of chromosomes. Consistent with these roles, it is found in the nucleus and on chromosomes, and it is located at the centrosome, in the nucleoplasm and on the spindle. The mouse ortholog is named Haspin.
The protein is also called haspin, and the gene has the alternative symbol GSG2. Both names come from an earlier designation, germ cell associated 2 (haspin). The current HGNC name, histone H3 associated protein kinase, describes the best-characterised function of the enzyme. Researchers studying histone H3 phosphorylation, centromere biology and the organisation of the mitotic chromosome often work with haspin because H3T3ph is the mark it deposits and the signal that guides the chromosomal passenger complex.
Haspin-mediated H3T3 phosphorylation supports normal progression through the cell cycle, making it a focus for studies of mitotic control.
Haspin may position and modulate AURKB and other CPC components at centromeres through H3T3ph.
The kinase helps ensure proper chromatid cohesion and metaphase alignment, relevant to chromosome segregation research.
Histone H3 Thr-3 phosphorylation is a defined mitotic histone mark, and haspin is the kinase annotated as responsible for it.
Haspin is a serine/threonine-protein kinase that phosphorylates histone H3 at Thr-3 during mitosis. This mark may help position and activate AURKB and other chromosomal passenger complex components at centromeres, supporting chromatid cohesion, metaphase alignment and normal cell cycle progression.
The official HGNC symbol is HASPIN, with GSG2 as an alternative symbol. The previous gene name was germ cell associated 2 (haspin). UniProt lists the protein as serine/threonine-protein kinase haspin, and the enzyme carries the EC number 2.7.11.1.
A mouse ortholog, Haspin, is recorded for the human gene. The protein belongs to the haspin subfamily of the Ser/Thr protein kinase family within the protein kinase superfamily. Check the datasheet of any product for its validated reactivity.
Chromosomal passenger complex components, histone H3 variants and other mitotic regulators with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8TF76; product specifications from Assay Genie datasheets.