IL-5
IL-5 (interleukin 5, also known as EDF and TRF) is a secreted homodimeric cytokine produced mainly by T-lymphocytes and NK cells. It regulates the survival, differentiation and chemotaxis of eosinophils and promotes B-cell growth and immunoglobulin production. Browse Assay Genie's current range of IL-5 research reagents below, or read on for the biology behind the target.
IL-5 is a homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells. Its central role is in the survival, differentiation and chemotaxis of eosinophils, and the NCBI Gene summary describes it as a major regulator of eosinophil formation, maturation, recruitment and survival. IL-5 also acts on activated and resting B-cells, where it induces growth, differentiation and immunoglobulin production.
IL-5 signals through a heterodimeric receptor made up of the IL5RA subunit and the cytokine receptor common subunit beta (CSF2RB). The beta subunit is shared with the receptors for IL-3 and GM-CSF (CSF2). Receptor binding activates kinases including LYN, SYK and JAK2, and the signal passes through the RAS-MAPK and JAK-STAT5 pathways. The protein belongs to the IL-5 family and is classed by HGNC in the interleukins gene group. Researchers working with the IL-5 protein study it as a secreted mediator, and the mouse and rat each have an orthologous Il5 gene.
The IL-5 gene (IL5) lies on chromosome 5q31 in a cytokine gene cluster that also contains IL4, IL13 and CSF2. According to the NCBI Gene summary, IL5, IL4 and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases. Increased IL-5 production may be related to the pathogenesis of eosinophil-dependent inflammatory diseases. The gene's earlier name was interleukin 5 (colony-stimulating factor, eosinophil), and the aliases EDF and TRF also appear in the literature.
IL-5 drives eosinophil formation, maturation, recruitment and survival, making it a central cytokine for studying how eosinophil populations are regulated.
Increased IL-5 production may be related to the pathogenesis of eosinophil-dependent inflammatory diseases, so it is a common focus in inflammation research.
IL-5 acts on activated and resting B-cells to induce growth, differentiation and immunoglobulin production.
IL-5 engages IL5RA and CSF2RB, activating LYN, SYK and JAK2 and signalling through the RAS-MAPK and JAK-STAT5 pathways.
IL-5 is a homodimeric cytokine made mainly by T-lymphocytes and NK cells. It supports the survival, differentiation and chemotaxis of eosinophils, and it induces growth, differentiation and immunoglobulin production in B-cells. It signals through a receptor of IL5RA and CSF2RB.
The HGNC symbol is IL5, and the aliases are EDF (eosinophil differentiation factor) and TRF. The earlier gene name was interleukin 5 (colony-stimulating factor, eosinophil). UniProt lists the protein as Interleukin-5, a member of the IL-5 family.
Mouse and rat both carry an orthologous Il5 gene, so rodent models are used to study it. Increased IL-5 production may be related to eosinophil-dependent inflammatory diseases. Check the datasheet of any product for its validated reactivity.
Other interleukins, including IL-13, which sits in the same chromosome 5q31 cytokine cluster, and further cytokines for immune signalling research.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P05113; product specifications from Assay Genie datasheets.