MMP12
MMP12, also known as macrophage metalloelastase or HME, is a secreted zinc-dependent endopeptidase of the matrix metalloproteinase family. It has significant elastolytic activity and is linked to tissue injury and remodelling. Browse Assay Genie's current range of MMP12 research reagents below, or read on for the biology behind the target.
MMP12 is a macrophage metalloelastase that acts in the extracellular space and extracellular matrix. It has significant elastolytic activity, and the mature protease degrades both soluble and insoluble elastin. It may be involved in tissue injury and remodelling. Its substrate preferences have been described at the cleavage site: it can accept large and small amino acids at the P1' position but prefers leucine, while aromatic or hydrophobic residues are favoured at P1 and small hydrophobic residues, preferably alanine, occupy P3.
The MMP12 gene encodes a preproprotein that is proteolytically processed to generate the mature protease. The protein belongs to the peptidase M10A family and sits within the M10 family of matrix metalloproteinases (MMPs), which HGNC groups with the M10 matrix metallopeptidases and the elastases. Members of this family contribute to the breakdown of extracellular matrix in normal physiological processes such as embryonic development, reproduction and tissue remodelling, and in disease processes such as arthritis and metastasis. The MMP12 gene lies within a cluster of MMP genes on chromosome 11. Mouse and rat both carry an Mmp12 ortholog.
The gene was previously named matrix metalloproteinase 12 (macrophage elastase). It may play a role in aneurysm formation, and mutations in the gene are associated with lung function and chronic obstructive pulmonary disease (COPD). These links make MMP12 a frequent subject in studies of elastin turnover and matrix degradation, from an MMP12 antibody used to localise the enzyme to an MMP12 ELISA kit used to measure it in biological fluids.
MMP12 degrades soluble and insoluble elastin, making it a key enzyme for studying elastolysis in the extracellular matrix.
Mutations in the MMP12 gene are associated with lung function and chronic obstructive pulmonary disease.
MMP12 may play a role in aneurysm formation, so it is studied in vascular matrix remodelling.
MMP12 may be involved in tissue injury and remodelling, alongside other MMPs that break down extracellular matrix.
MMP12 is a secreted metalloelastase with significant elastolytic activity. The mature protease degrades soluble and insoluble elastin in the extracellular matrix, and it may be involved in tissue injury and remodelling. It prefers leucine at the P1' site of its substrates.
MMP12 is also called macrophage metalloelastase and HME. Its previous HGNC name was matrix metalloproteinase 12 (macrophage elastase). The enzyme is classified as EC 3.4.24.65 and belongs to the peptidase M10A family of matrix metalloproteinases.
Mutations in the MMP12 gene are associated with lung function and COPD, and the gene may play a role in aneurysm formation. Orthologs named Mmp12 exist in mouse and rat; check the datasheet of any product for its validated reactivity.
Other matrix metalloproteinases involved in extracellular matrix breakdown and tissue remodelling.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P39900; product specifications from Assay Genie datasheets.