G6PD
G6PD (glucose-6-phosphate dehydrogenase, also written G6PD1) is a cytosolic oxidoreductase that catalyses the rate-limiting step of the oxidative pentose-phosphate pathway. Its main output is NADPH, the reducing power used in antioxidant defence and reductive biosynthesis. Browse Assay Genie's current range of G6PD research reagents below, or read on for the biology behind the target.
G6PD catalyses the rate-limiting step of the oxidative pentose-phosphate pathway, a route for the dissimilation of carbohydrates besides glycolysis. The main function of the enzyme is to provide reducing power in the form of NADPH, together with pentose phosphates for fatty acid and nucleic acid synthesis. NADPH is a key electron donor in the defence against oxidising agents and in reductive biosynthetic reactions. UniProt also records that G6PD converts NAADPH back to NAADP. NAADPH is produced from NAADP by enzymes such as DUOX1, DUOX2 and NOX5, and NAADP promotes Ca(2+) signalling during T cell activation.
The G6PD gene is a housekeeping gene on the X chromosome, and the protein is a cytosolic enzyme; UniProt lists cytosol and membrane as subcellular locations. The protein belongs to the glucose-6-phosphate dehydrogenase family and is classified by HGNC in the oxidoreductases gene group. NCBI Gene reports two transcript variants that encode different isoforms. The mouse and rat orthologues are both named G6pdx, which is useful when selecting a model for G6PD protein studies.
G6PD is notable for its genetic diversity. Many variants, mostly produced by missense mutations, have been described, with a wide range of enzyme activity levels and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute haemolysis, or severe chronic non-spherocytic haemolytic anaemia. This variability makes the G6PD gene a frequent subject in studies of redox balance and red cell biology.
G6PD supplies NADPH, a key electron donor in defence against oxidising agents, so it is central to studies of cellular redox balance.
As the rate-limiting enzyme of the oxidative pentose-phosphate pathway, G6PD is a focal point for work on carbohydrate flux outside glycolysis.
G6PD deficiency may cause neonatal jaundice, acute haemolysis or chronic non-spherocytic haemolytic anaemia, making the enzyme relevant to red cell disease research.
G6PD converts NAADPH back to NAADP, which promotes Ca(2+) signalling during T cell activation, linking metabolism to immune signalling.
G6PD catalyses the rate-limiting step of the oxidative pentose-phosphate pathway. Its main role is to provide NADPH and pentose phosphates for fatty acid and nucleic acid synthesis. It also converts NAADPH back to NAADP, which promotes Ca(2+) signalling during T cell activation.
The HGNC symbol is G6PD, with G6PD1 as an alias, and the full name is glucose-6-phosphate dehydrogenase. UniProt names the protein glucose-6-phosphate 1-dehydrogenase. NCBI Gene reports two transcript variants that encode different isoforms.
G6PD is highly variable, with many mostly missense variants affecting enzyme activity. G6PD deficiency may cause neonatal jaundice, acute haemolysis or chronic non-spherocytic haemolytic anaemia. Mouse and rat orthologues are both called G6pdx; check the datasheet of any product for its validated reactivity.
Other NADPH oxidases, pentose-phosphate pathway enzymes and redox-related targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P11413; product specifications from Assay Genie datasheets.