SNAP25
SNAP25 (synaptosome associated protein 25, also known as SNAP-25, RIC-4 or SEC9) is a t-SNARE protein involved in the molecular regulation of neurotransmitter release. It is a presynaptic plasma membrane protein that contributes to vesicle docking and membrane fusion. Browse Assay Genie's current range of SNAP25 research reagents below, or read on for the biology behind the target.
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) on the vesicle membrane (v-SNAREs) and on the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex is a bundle of four helices: one supplied by the v-SNARE and three by the t-SNARE. For t-SNAREs at the plasma membrane, syntaxin supplies one helix and SNAP25 contributes the other two. The protein therefore acts in the regulation of neurotransmitter release and associates with proteins involved in vesicle docking and membrane fusion.
Beyond its core role in exocytosis, SNAP25 may be important for the synaptic function of specific neuronal systems. It regulates plasma membrane recycling through its interaction with CENPF, and it modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells. UniProt records its location as the perinuclear cytoplasm, the cell membrane, the synaptosome and the photoreceptor inner segment.
The SNAP25 gene belongs to the SNAP-25 family and to the HGNC SNAREs gene group. Two alternative transcript variants encoding different protein isoforms have been described. Previous names include synaptosomal-associated protein, 25kDa, and further aliases include RIC4, bA416N4.2, dJ1068F16.2 and SNAP. Mouse and rat both carry a Snap25 ortholog, which supports comparative work on the SNAP25 protein in standard model systems.
SNAP25 is a t-SNARE that regulates neurotransmitter release, making it a central target for studying presynaptic vesicle function.
SNAP25 contributes two helices to the four-helix SNARE bundle formed with syntaxin and a v-SNARE, which drives membrane fusion.
SNAP25 regulates plasma membrane recycling through its interaction with CENPF, linking SNARE biology to membrane trafficking.
SNAP25 modulates gating of the voltage-dependent potassium channel KCNB1 in pancreatic beta cells, extending its relevance beyond neurons.
SNAP25 is a t-SNARE on the presynaptic plasma membrane. It supplies two of the four helices in the SNARE complex, alongside syntaxin and a vesicle v-SNARE, and so helps regulate neurotransmitter release. It also modulates the potassium channel KCNB1 in pancreatic beta cells.
SNAP25 is also written SNAP-25 and is known as RIC-4, RIC4, SEC9, bA416N4.2, dJ1068F16.2 and SNAP. The previous HGNC name was synaptosomal-associated protein, 25kDa. Two alternative transcript variants encoding different protein isoforms have been described for the gene.
Yes. Mouse and rat each have an ortholog named Snap25, so the protein can be studied in common rodent models. As with any reagent, check the datasheet of any product for its validated reactivity before use.
Other SNARE and SNAP-25 family proteins and related synaptic trafficking targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P60880; product specifications from Assay Genie datasheets.