3D Cell Culture Ready-to-Use Scaffold Complete Kit (BN01148)

SKU:
BN01148
Product type:
Cellular Assay
  • Cell Health Assays 3D Cell Culture Ready-to-Use Scaffold Complete Kit BN01148
  • Cell Health Assays 3D Cell Culture Ready-to-Use Scaffold Complete Kit BN01148
  • Cell Health Assays 3D Cell Culture Ready-to-Use Scaffold Complete Kit BN01148
  • Cell Health Assays 3D Cell Culture Ready-to-Use Scaffold Complete Kit BN01148
  • Cell Health Assays 3D Cell Culture Ready-to-Use Scaffold Complete Kit BN01148
  • Cell Health Assays 3D Cell Culture Ready-to-Use Scaffold Complete Kit BN01148
  • Cell Health Assays 3D Cell Culture Ready-to-Use Scaffold Complete Kit BN01148
  • Cell Health Assays 3D Cell Culture Ready-to-Use Scaffold Complete Kit BN01148
  • Cell Health Assays 3D Cell Culture Ready-to-Use Scaffold Complete Kit BN01148
€703

Description

ELISA Kit Technical ManualMSDS

3D Cell Culture Ready-to-Use Scaffold Complete Kit

Three dimensional (3D) cell cultures are artificially-created environments in which cells are permitted to grow or interact with their surroundings in a 3D fashion. 3D cell culture environments improve the function, differentiation and viability of cells and recapitulate in vivo microenvironment compared to conventional 2D cell culture experiments. 3D matrices and scaffolds provide a physiologically relevant screening platform, by mimicking the in vivo responses, for many cell types including cancer and stem cells in developmental morphogenesis, pharmacology, drug metabolism and drug toxicity studies. Assay Genie offers 3D cell culture kits, including Basement Membrane Matrix (BME, animal-based), Alginate Hydrogel (plant-based), proprietary Assay Genie's Duo-Matrix and dry Scaffold (natural polymers, animal-free) to meet the needs and requirements of various research fields. Assay Genie's 3D Cell Culture Ready-to-Use Scaffold Complete Kit provides a standardized, and adaptable to high-throughput strategy microplate containing proprietary 3D scaffolds for setting up spheroid formations, 3D cell cultures, pharmacological studies, with an optimized scaffold dissociation method for subsequent biochemical or protein analysis

Figure: HepG2 and MCF7 cells in No Scaffold (A) and 3D Scaffold (B). Cells were cultured in Scaffolds for 21 days, and successfully formed spheroids. Media was changed every 2-3 days as per protocol. The Calcein AM staining (C) indicates that cell viability is not affected while culturing in Scaffold for a long period of time. (D) Image of Ready-to-Use dry Scaffold sponges in a 96 well plate. (E) Dry Scaffold without media or cells: high magnification image from light microscopy shows the interconnected pores (50-200 µm in size) within the scaffold. (F) Scaffold dissociation (step VII-3) was carried out on HEPG2 cells, grown in Scaffold for 21 days. There is no significant cell death (Red: Ethidium Homodimer-1) after dissociation. Viable cells are shown in green (Calcein AM). Note: Ethidium Homodimer-1 and Calcein AM are not included in the kit.

Key Info Description

Product SKU

BN01148

Size

100 assays

Detection Method

Qualitative

Species Reactivity

Mammalian

Applications

Spheroid Formation Assays

Features & Benefits

Reproducible, Qualitative tool for screening, studying, and characterizing compounds in cell culture studies

Kit Components

  • Scaffold Dissociation Solution (enzyme-free) Neutralization Buffer
  • Ready-to-Use Scaffolds (96-well plate)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

Usage

For Research Use Only! Not For Use in Humans.

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